FlyBase Allele Report: Dmel\Lk[GAL4::VP16]

General Information

Symbol

Dmel\Lk^GAL4::VP16

Species

D. melanogaster

Name

FlyBase ID

FBal0344968

Feature type

allele

Associated gene

Dmel\Lk

Associated Insertion(s)

TI{GAL4::VP16}Lk^GAL4::VP16

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Zandawala et al., 2018)

Mutagen

gene targeting by homologous recombination

(Zandawala et al., 2018)

CRISPR/Cas9

(Zandawala et al., 2018)

Progenitor genotype

Associated Insertion(s)

TI{GAL4::VP16}Lk^GAL4::VP16

Cytology

Description

GAL4::VP16 and a Disc\RFP^3xP3.cUa marker flanked by loxP sites is inserted in Lk immediately following the ATG translational start site (+4bp to +10bp, relative to start site).

(Zandawala et al., 2018)

Allele components

Component

Use(s)

Inserted element

TI{GAL4::VP16}

promoter trap

Encoded product / tool

GAL4::VP16

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage

(Zandawala et al., 2018)

abnormal proboscis extension reflex | adult stage | dominant

(Zandawala et al., 2018)

abnormal starvation stress response | adult stage | dominant

(Zandawala et al., 2018)

abnormal stress response | adult stage | dominant

(Zandawala et al., 2018)

decreased rate of movement | adult stage | dominant

(Zandawala et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Lk^GAL4::VP16 homozygous and heterozygous mutant adults survive significantly longer under starvation or desiccation stress, compared to controls; they have significantly reduced proboscis extensions in response to tarsal sucrose, as well as significantly reduced long-term, but not short-term food intake, compared to controls, with a stronger effect in homozygotes; they have significantly reduced locomotor activity and metabolic rate (carbon dioxide consumption), compared to controls.

(Zandawala et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Lk^GAL4::VP16 is partially rescued by Lk^UAS.cZa/Scer\GAL4^{Lk-GAL4::VP16}

(Zandawala et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Lk^CC9

(Savaş et al., 2026)

Lk^GAL4::VP16

Lk^GAL4

(Puri et al., 2026)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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