FlyBase Allele Report: Dmel\SH3PX1[d1]

General Information

Symbol

Dmel\SH3PX1^d1

Species

D. melanogaster

Name

FlyBase ID

FBal0349900

Feature type

allele

Associated gene

Dmel\SH3PX1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhang et al., 2019)

Mutagen

P-element activity

(Zhang et al., 2019)

Progenitor genotype

P{GSV7}GS20844

(Zhang et al., 2019)

Cytology

Description

1125bp deletion that includes the start codon and first exon of SH3PX1.

(Zhang et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Zhang et al., 2019)

CEA with SH3PX1^HK62b

(Zhang et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of colorectal cancer

is ameliorated by Hsap\SNX18^UAS.Tag:V5

(Zhang et al., 2019)

is ameliorated by Hsap\SNX33^UAS.Tag:V5

(Zhang et al., 2019)

is ameliorated by Hsap\SNX9^UAS.Tag:V5

(Zhang et al., 2019)

is ameliorated by Krn^NIG.8056R

(Zhang et al., 2019)

is ameliorated by rho^JF03106

(Zhang et al., 2019)

is ameliorated by shi^1.UAS

(Zhang et al., 2019)

is ameliorated by yki^KK109756

(Zhang et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Zhang et al., 2019)

increased cell number | adult stage (with SH3PX1^HK62b)

(Zhang et al., 2019)

increased occurrence of cell division | adult stage | recessive

(Zhang et al., 2019)

short lived | recessive

(Zhang et al., 2019)

Phenotype Manifest In

adult midgut epithelium

(Zhang et al., 2019)

adult midgut epithelium (with SH3PX1^HK62b)

(Zhang et al., 2019)

adult midgut epithelium | somatic clone

(Zhang et al., 2019)

autophagosome | adult stage | somatic clone

(Zhang et al., 2019)

enteroblast | adult stage

(Zhang et al., 2019)

intestinal stem cell | adult stage

(Zhang et al., 2019)

Detailed Description

Statement

Reference

7 days old SH3PX1^d1 homozygotes display severely increased numbers of progenitor cells (Mira-positive intestinal stem cells and enteroblasts) and of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to heterozygous and wild-type controls. Likewise, SH3PX1^d1/SH3PX1^HK62b transheterozygotes also show a significant increase in the number of mitotic cells (phospho-H3 staining), as compared to SH3PX1^d1 heterozygous controls.

SH3PX1^d1 homozygous midgut clones generated during adulthood are significantly larger than control clones, but present similar frequencies of enterocytes (Pdm1-positive) and enteroendocrine cells (Prospero-positive), as compared to controls. The clonal cells fail to show the expected starvation-induced autophagosome formation (identified as Atg8-positive puncta) normally observed in controls.

(Zhang et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SH3PX1^d1 has increased occurrence of cell division | recessive | adult stage phenotype, suppressible | partially by upd3^Δ/upd2^Δ

(Zhang et al., 2019)

SH3PX1^d1 has increased occurrence of cell division | recessive | adult stage phenotype, suppressible | partially by Hsap\SNX9^UAS.Tag:V5/Scer\GAL4^esg.PU