FlyBase Allele Report: Dmel\iPLA2-VIA[null]

General Information

Symbol

Dmel\iPLA2-VIA^null

Species

D. melanogaster

Name

FlyBase ID

FBal0355562

Feature type

allele

Associated gene

Dmel\iPLA2-VIA

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Mori et al., 2019)

Mutagen

CRISPR/Cas9

(Mori et al., 2019)

Progenitor genotype

Cytology

Description

Deletion of iPLA2-VIA.

(Mori et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:9,860,815..9,864,100 [-]

Comment:

The iPLA2-VIA^null deletion ranges from intron 1 to exon 8 of iPLA2-VIA-RB and removes most of the CDS.

(Imai and Hattori, 2020.2.4)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 14

CEA

(Mori et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease 14

is ameliorated by CG3740^EY03391

(Mori et al., 2019)

is ameliorated by Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

is ameliorated by Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

iPLA2-VIA^null homozygous adults show a severe and progressive decrease in climbing activity, disturbance in sleep, namely a significant increase in day time sleep and a significant and progressive decrease in night time sleep, and heat sensitivity. Their brains show vacuolization, a progressive decrease in the number of DA neurons (PPL1, PPM1/2 and PPM3), and ER stress. In light microscopy of DA neurons and indirect flight muscles, mitochondrial morphology appears normal. In TEM of the adult brain, there are aberrant crista dilation, multilamellar bodies and highly electron-dense structures.

iPLA2-VIA^null homozygous third instar larval show poorly-developed salivary glands with extended ER and signs of ER stress, as compared to controls. Their neuromuscular junctions show more and smaller synaptic vesicles, despite of no changes in docked vesicle number, bouton number and size or activation zone number. Neuromuscular neurotransmission shows a significant decrease in mEJP amplitude at 22[o]C and 30[o]C, but no major changes in EJP amplitude and quantal content, as compared to controls; however, PPR is reduced at 30[o]C, but not at 22[o]C, as compared to controls.

(Mori et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

iPLA2-VIA^null has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal sleep | progressive phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has decreased cell number | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal heat stress response phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has short lived | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal heat stress response phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has short lived | heat sensitive phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, suppressible | partially by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal heat stress response phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has short lived | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has decreased cell number | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has decreased cell number | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

NOT suppressed by

Statement

Reference

iPLA2-VIA^null has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has short lived phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\C19orf12^G69R.UAS

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsc70-3^K97S.UAS

(Mori et al., 2019)

iPLA2-VIA^null has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has abnormal sleep | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has bang sensitive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has decreased cell number | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has decreased cell number | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

iPLA2-VIA^null has dopaminergic PPL1 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM1 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM2 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM3 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has adult brain phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPL1 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM1 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM2 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM3 neuron | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Hsc70-3^UAS.cEa

(Mori et al., 2019)

iPLA2-VIA^null has synaptic vesicle | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/CG3740^EY03391

(Mori et al., 2019)

iPLA2-VIA^null has adult brain phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has endoplasmic reticulum | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has embryonic/larval salivary gland | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has endoplasmic reticulum | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has endoplasmic reticulum | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has endoplasmic reticulum | adult stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has endoplasmic reticulum | adult stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has synaptic vesicle | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has synaptic vesicle | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^UAS.cMa

(Mori et al., 2019)

iPLA2-VIA^null has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\C19orf12^UAS.cMa

(Mori et al., 2019)

NOT suppressed by

Statement

Reference

iPLA2-VIA^null has adult brain phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPL1 neuron | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM1 neuron | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM2 neuron | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has dopaminergic PPM3 neuron | progressive phenotype, non-suppressible by Scer\GAL4^elav-C155/Scer\TOM70::Scer\UBC6^UAS.GFP

(Mori et al., 2019)

iPLA2-VIA^null has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\PLA2G6^A80T.UAS

(Mori et al., 2019)

iPLA2-VIA^null has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\C19orf12^G69R.UAS

(Mori et al., 2019)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The extended ER observed in the salivary glands of iPLA2-VIA^null third instar larvae is suppressed by the Scer\GAL4^elav-C155-driven expression of Hsap\C19orf12^UAS.cMa or Hsap\PLA2G6^UAS.cMa, even leading to a decrease in ER area.

(Mori et al., 2019)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

diPLA2-VIA^null

(Mori et al., 2019)

iPLA2-VIA^null

(Imai and Hattori, 2020.2.4)

Name Synonyms

Secondary FlyBase IDs

References (2)

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