FlyBase Allele Report: Scer\GAL4[Eh-pan-Gal4]

General Information

Symbol

Scer\GAL4^Eh-pan-Gal4

Species

S. cerevisiae

Name

FlyBase ID

FBal0360742

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{2A-GAL4}Eh^pan-Gal4

Carried in Construct

Also Known As

Eh^pan-Gal4

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Scott et al., 2020)

Progenitor genotype

TI{T-GEM.0}Eh^Gal4

(Scott et al., 2020)

Associated Insertion(s)

TI{2A-GAL4}Eh^pan-Gal4

Cytology

Description

The RMCE cassette in TI{T-GEM.0}Eh^Gal4 has been replaced with sequence encoding Eh third intron sequence (from 3' to the original insertion site) and the coding sequence of Eh exon 4, following by T2A-GAL4. This results in the reconstitution of full-length Eh coding sequence, and the presence of the T2A sequence allows separate translation of the GAL4 driver under the control of endogenous Eh regulatory sequences.

(Scott et al., 2020)

Allele components

Component

Use(s)

Inserted element

TI{2A-GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval anterior spiracle

(Scott et al., 2020)

embryonic/larval posterior spiracle

(Scott et al., 2020)

Keilin organ | surrounding

(Scott et al., 2020)

mouthpart

(Scott et al., 2020)

embryonic/larval tracheal dorsal trunk

(Scott et al., 2020)

larval Eh neurosecretory neuron

(Scott et al., 2020)

pharate adult stage

mouthpart

(Scott et al., 2020)

air sac primordium

(Scott et al., 2020)

adult central brain | restricted

(Scott et al., 2020)

Additional Information

Statement

Reference

Reporter expression is observed in the EH neurosecretory neurons in L1, L2 and L3 and in additional neurons in L3. Labeling is consistantly observed in all larval stages in the anterior and posterior spiracles and in cells associated with the mouthparts. In addition to spiracle and mouthpart labeling, expression at L1/L2 includes epithelial cells of the dorsal tracheal trunks, numerous superficial cells along the ventral and lateral body wall, and cells in segments T1 to T3 surrounding Keilin’s organs. Cells on the segmental branches of the dorsal tracheal trunks are also weakly labeled. In pharate adults, expression is observed in presumptive head and thoracic air sacs.

(Scott et al., 2020)

Marker for

Reflects expression of

(Scott et al., 2020)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Eh-pan-Gal4

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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