FlyBase Allele Report: Dmel\Tom70[miniGAL4Δ]

General Information

Symbol

Dmel\Tom70^miniGAL4Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0361260

Feature type

allele

Associated gene

Dmel\Tom70

Associated Insertion(s)

TI{miniGAL4(2)}Tom70^miniGAL4Δ

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Dutta et al., 2020)

Mutagen

CRISPR/Cas9

(Dutta et al., 2020)

gene targeting by homologous recombination

(Dutta et al., 2020)

Progenitor genotype

Associated Insertion(s)

TI{miniGAL4(2)}Tom70^miniGAL4Δ

Cytology

Description

The coding sequence of the endogenous Tom70 locus has been replaced with sequence encoding miniGAL4(2) and a dominant marker, allowing expression of miniGAL4(2) under the control of endogenous regulatory sequences.

(Dutta et al., 2020)

Allele components

Component

Use(s)

Inserted element

TI{miniGAL4(2)}

promoter trap

Encoded product / tool

miniGAL4(2)

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of mitochondrial metabolism disease

CEA

(Dutta et al., 2020)

CEA with Hsap\TOMM70^{I554F.UAS.Tag:HA}

(Dutta et al., 2020)

CEA with Hsap\TOMM70^{T607I.UAS.Tag:HA}

(Dutta et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage

(Dutta et al., 2020)

lethal - all die before end of pupal stage (with Df(2L)Exel6031)

(Dutta et al., 2020)

majority die during larval stage

(Dutta et al., 2020)

some die during pupal stage

(Dutta et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Tom70^miniGAL4Δ has increased mortality during development phenotype, suppressible by Hsap\TOMM70^UAS.Tag:HA/Scer\GAL4^{Tom70-miniGAL4Δ}

(Dutta et al., 2020)

Tom70^miniGAL4Δ has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^{Tom70-miniGAL4Δ}/Hsap\TOMM70^{I554F.UAS.Tag:HA}

(Dutta et al., 2020)

Tom70^miniGAL4Δ has increased mortality during development phenotype, suppressible | partially by Hsap\TOMM70^{T607I.UAS.Tag:HA}/Scer\GAL4^{Tom70-miniGAL4Δ}

(Dutta et al., 2020)

Other

Statement

Reference

Hsap\TOMM70^{I554F.UAS.Tag:HA}, Scer\GAL4^{Tom70-miniGAL4Δ}, Tom70^miniGAL4Δ has partially lethal - majority die phenotype

(Dutta et al., 2020)

Hsap\TOMM70^{T607I.UAS.Tag:HA}, Scer\GAL4^{Tom70-miniGAL4Δ}, Tom70^miniGAL4Δ has partially lethal - majority die phenotype

(Dutta et al., 2020)

Hsap\TOMM70^UAS.Tag:HA, Scer\GAL4^{Tom70-miniGAL4Δ}, Tom70^miniGAL4Δ has viable phenotype

(Dutta et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tom70^miniGAL4Δ

(Dutta et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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