FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\SvipKO
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General Information
Symbol
Dmel\SvipKO
Species
D. melanogaster
Name
FlyBase ID
FBal0367205
Feature type
allele
Associated gene
Dmel\Svip
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Johnson et al., 2021)
Mutagen
CRISPR/Cas9
(Johnson et al., 2021)
Progenitor genotype
Cytology
Description

2bp deletion within the first exon of Svip; this results in a frame-shift and a premature stop codon upstream of the VCP-interaction motif.

(Johnson et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:9,359,174..9,359,175 [+]
Comment:

A 2bp deletion in the first exon that results in a frameshift and premature stop codon.

(Johnson et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  syndrome
      CEA
      (Johnson et al., 2021)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      SvipKO homozygosity leads to fragmentation of the lysosome tubule network in body wall muscles of third instar larvae, as well as in adult abdominal muscles: decreased number of junctions per tubule and decreased tubule density.

      SvipKO homozygous adults show a progressive decrease in climbing activity; aged adults show shorter neuromuscular junctions, which have a darkened appearance with large vesicles; abdominal muscles show signs of wasting, impaired mitochondria morphology and hallmarks of neuromuscular synapse degeneration (less striated t-tubules and disorganized Z-bands, nuclei are variably positioned, mitochondria are altered and with apparent vacuoles).

      TTM motoneurons show progressive degeneration from pupal development through adult life, with a progressive decrease in dendrite length and volume; TTM and DLM motoneuron neuromuscular junction recordings show failure in aged adults. Aged adults show increased apoptosis in the ventral nerve cord, optic lobe and neural circuitry of the head.

      (Johnson et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      SvipKO heterozygous third instar larvae expressing SvipS82L.UAS under the control of Scer\GAL4Mhc.PU show fragmentation of the lysosome tubule network: decreased number of junctions per tubule and decreased tubule density.

      (Johnson et al., 2021)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      SvipKO is partially rescued by SvipUAS.cJa/Scer\GAL4Mhc.PU

      (Johnson et al., 2021)
      Not rescued by

      SvipKO is not rescued by SvipRR.UAS.mCherry/Scer\GAL4Mhc.PU

      (Johnson et al., 2021)

      SvipKO is not rescued by Scer\GAL4unspecified/SvipUAS.cJa

      (Johnson et al., 2021)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      SVIPKO
      (Johnson et al., 2021)
      SvipKO
      Name Synonyms
      Secondary FlyBase IDs
        References (1)