FlyBase Allele Report: Dmel\mim[CR70074-TG4.0]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\mim^{CR70074-TG4.0}

General Information

Symbol

Dmel\mim^{CR70074-TG4.0}

Species

D. melanogaster

Name

FlyBase ID

FBal0368528

Feature type

allele

Associated gene

Associated Insertion(s)

TI{CRIMIC.TG4.0}mim^{CR70074-TG4.0}

Carried in Construct

Key Links

Genomic Maps

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.0}mim^{CR70074-TG4.0}

Cytology

Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into mim, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TTAAAGGTCCTAGTTGTCAGTGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.0}

RMCE target element

Encoded product / tool

binary expression system - driver

Also carries

integrase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

2R:6,934,269..6,934,270 [-]

(Huang et al., 2022, Kanca et al., 2022, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual developmental disorder with ocular anomalies and distinctive facial features

CEC

(Huang et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intellectual developmental disorder with ocular anomalies and distinctive facial features

is ameliorated by Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage (with Df(2R)Exel6051)

(Huang et al., 2022)

bang sensitive | adult stage (with Df(2R)Exel6051)

(Huang et al., 2022)

decreased rate of adult walking behavior (with Df(2R)Exel6051)

(Huang et al., 2022)

partially lethal - majority die

(Huang et al., 2022)

short lived (with Df(2R)Exel6051)

(Huang et al., 2022)

viable (with Df(2R)Exel6051)

(Huang et al., 2022)

Phenotype Manifest In

retina | adult stage (with Df(2R)Exel6051)

(Huang et al., 2022)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has bang sensitive | adult stage phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^R671W.UAS

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

mim^{CR70074-TG4.0} has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has short lived phenotype, suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has short lived phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^R671W.UAS

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has decreased rate of adult walking behavior phenotype, suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has bang sensitive | adult stage phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

NOT suppressed by

Statement

Reference

mim^{CR70074-TG4.0} has partially lethal - majority die phenotype, non-suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^R671W.UAS

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has decreased rate of adult walking behavior phenotype, non-suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^R671W.UAS

(Huang et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has retina | adult stage phenotype, suppressible | partially by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^UAS.cBa

(Huang et al., 2022)

NOT suppressed by

Statement

Reference

mim^{CR70074-TG4.0}/Df(2R)Exel6051 has retina | adult stage phenotype, non-suppressible by Scer\GAL4^{mim-CR70074-TG4.0}/Hsap\MTSS2^R671W.UAS

(Huang et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

92698

y¹ w^*; TI{CRIMIC.TG4.0}mim^{CR70074-TG4.0}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mim^{CR70074-TG4.0}

mim^T2A-GAL4

(Huang et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections