FB2026_02 , released June 18, 2026
Allele: Scer\GAL4mim-CR70074-TG4.0
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General Information
Symbol
Scer\GAL4mim-CR70074-TG4.0
Species
S. cerevisiae
Name
FlyBase ID
FBal0368606
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{CRIMIC.TG4.0}mimCR70074-TG4.0
Carried in Construct
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{CRIMIC.TG4.0}mimCR70074-TG4.0
Cytology
Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into mim, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TTAAAGGTCCTAGTTGTCAGTGG.

(Gene Disruption Project members, 2018-)
Allele components
Component
Use(s)
Inserted element
TI{CRIMIC.TG4.0}
Encoded product / tool
GAL4
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval brain
      (Huang et al., 2022)
      ventral nerve cord | restricted
      (Huang et al., 2022)
      larval ventral nerve cord
      (Huang et al., 2022)
      adult stage
      adult brain
      (Huang et al., 2022)
      mushroom body
      (Huang et al., 2022)
      adult optic lobe
      (Huang et al., 2022)
      adult mushroom body
      (Huang et al., 2022)
      adult central brain
      (Huang et al., 2022)
      adult ventral nerve cord
      (Huang et al., 2022)
      Additional Information
      Statement
      Reference

      In the larval CNS, expression is observed primarily in neurons and in some glia in the ventral nerve cord. In the adult brain, expression is expressed in most neurons and many glia of the central brain and optic lobe. It is highly expressed in neurons of the mushroom body.

      (Huang et al., 2022)

      mim is highly expressed in most neurons and in some glia in the larval ventral nerve cord and in adult mushroom body, central brain, optic lobe, and ventral nerve cord.

      (Huang et al., 2022)
       
      Marker for
      Reflects expression of
      mim
      (Huang et al., 2022)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4mim-CR70074-TG4.0
      mimT2A-GAL4
      (Huang et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)