A TI{CRIMIC.TG4.0} DNA cassette has been inserted into tau, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: AGATTTATGCCCATTATAGATGG.
tauCR70012-TG4.0, Hsap\MAPT1xUAS.0N4R.EGFP, Scer\GAL4tau-CR70012-TG4.0 is a non-enhancer of lipid droplet | adult stage | increased number phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPT1xUAS.0N4R.EGFP, Scer\GAL4tau-CR70012-TG4.0 is a non-enhancer of ommatidium | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPTA152T.1xUAS.2N4R, Scer\GAL4tau-CR70012-TG4.0 is a suppressor of lipid droplet | increased number | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPTA152T.1xUAS.2N4R, Scer\GAL4tau-CR70012-TG4.0 is a suppressor of ommatidium | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPTR406W.1xUAS.2N4R, Scer\GAL4tau-CR70012-TG4.0 is a suppressor of lipid droplet | increased number | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Scer\GAL4tau-CR70012-TG4.0, Hsap\MAPTP301S.1xUAS.2N4R is a suppressor of lipid droplet | increased number | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Scer\GAL4tau-CR70012-TG4.0, Hsap\MAPTP301S.1xUAS.2N4R is a suppressor of ommatidium | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPTR406W.1xUAS.2N4R, Scer\GAL4tau-CR70012-TG4.0 is a suppressor of ommatidium | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPT1xUAS.0N4R.EGFP, Scer\GAL4tau-CR70012-TG4.0 is a non-suppressor of lipid droplet | increased number | adult stage phenotype of ND-42ninaE.GD6220
tauCR70012-TG4.0, Hsap\MAPT1xUAS.0N4R.EGFP, Scer\GAL4tau-CR70012-TG4.0 is a non-suppressor of ommatidium | adult stage phenotype of ND-42ninaE.GD6220