Incomplete homologous recombination at the jbug locus, resulting in part of the donor construct being inserted into the locus. Recombination of the 3' homology arm occurred as expected, but the other homology arm has been inserted into the jbug locus along with a loxP cassette containing a w+mW.hs marker. The insertion disrupts the jbug-RI and jbug-RF/M/N isoforms.
decreased size | adult stage (with jbug20)
adult thorax (with Df(2R)59AB)
adult thorax (with jbug20)
macrochaeta (with Df(2R)59AB)
macrochaeta (with jbug20)
jbug30/jbug30, jbug30/Df(2R)59AB and jbug30/jbug20 adult flies produce short, bent, and rough-ended bristles on the thorax; jbug30/jbug30 pupae also show bent thoracic bristles. jbug30/jbug30 adults also exhibit a mild swirling pattern of wing hairs; hair formation is delayed in pupal wings.
jbug30/jbug30 stage 16 embryos show fewer but relatively normal denticles.
jbug30 is partially rescued by jbugUAS.RF/Scer\GAL4da.G32
jbug30 is partially rescued by jbugUAS.RF/Scer\GAL469B
jbug30 is partially rescued by jbugUAS.RI/Scer\GAL4da.G32