FB2026_02 , released June 18, 2026
Allele: Dmel\DroncKO
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General Information
Symbol
Dmel\DroncKO
Species
D. melanogaster
Name
FlyBase ID
FBal0373181
Feature type
allele
Associated gene
Dmel\Dronc
Associated Insertion(s)
TI{TI}DroncKO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Arthurton et al., 2020)
Mutagen
CRISPR/Cas9
(Arthurton et al., 2020)
recombination
(Arthurton et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{TI}DroncKO
Cytology
Description

Replacement of part of the 5' UTR and the whole first exon of Dronc with an attP-integration site, creating a knock-out allele.

(Arthurton et al., 2020)
Allele components
Component
Use(s)
Also carries
attP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3L:9,968,861..9,969,523 [+]
Comment:

Replacement of part of the 5' UTR and the whole first exon of Dronc with an attP-integration site.

(Baena-lopez, 2022.3.26)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      fertile (with DroncKO.Dronc.WT.SunTag(Unk),Tag:HA)

      (Arthurton et al., 2020)

      hyperplasia | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.CA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      hyperplasia | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.CAEA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      hyperplasia | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.deltaCAEA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      hyperplasia | adult stage | somatic clone - tissue specific (with DroncKO.-FRT.QF)

      (Arthurton et al., 2020)

      hyperplasia | adult stage | somatic clone - tissue specific (with DroncKO.-FRT.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      increased cell size | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.CA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      increased cell size | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.CAEA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      increased cell size | adult stage | somatic clone - tissue specific (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.Dronc.deltaCAEA.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      increased cell size | adult stage | somatic clone - tissue specific (with DroncKO.-FRT.QF)

      (Arthurton et al., 2020)

      increased cell size | adult stage | somatic clone - tissue specific (with DroncKO.-FRT.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)

      increased occurrence of cell division | adult stage | somatic clone - tissue specific (with DroncKO.-FRT.QF)

      (Arthurton et al., 2020)

      lethal - all die before end of pupal stage

      (Arthurton et al., 2020)

      lethal - all die before end of pupal stage (with DroncI29)

      (Arthurton et al., 2020)

      viable (with DroncKO.Dronc.WT.SunTag(Unk),Tag:HA)

      (Arthurton et al., 2020)

      viable (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.QF)

      (Arthurton et al., 2020)

      viable (with DroncKO.FRT.Dronc.WT.GFP.APEX2.FRT.SunTag(Unk),Tag:HA,mCherry)

      (Arthurton et al., 2020)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      DroncKO.-FRT.QF/DroncKO transheterozygous clones specifically generated in adult intestinal stem cells/enteroblasts, leads to sings of hyperplasia in the posterior midgut, with increased size of esg-positive progenitors (ISCs and EBs), and increased proportion of proliferating cells.

      DroncKO.-FRT.SunTag(Unk),Tag:HA,mCherry/DroncKO transheterozygous clones specifically generated in adult intestinal stem cells/enteroblasts, leads to sings of hyperplasia in the posterior midgut, with increased number and size of esg-positive progenitors (ISCs and EBs), and increased number of enterocytes.

      (Arthurton et al., 2020)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Adult posterior midgut clones specifically generated in esg-positive progenitors (ISCs and EBs), and which both express NdsRNA.UAS.cUa under the control of Scer\GAL4esg.PU and are DroncKO.-FRT.SunTag(Unk),Tag:HA,mCherry/DroncKO transheterozygous show increased proportion of progenitors.

      Adult posterior midgut clones specifically generated in esg-positive progenitors (ISCs and EBs), and which both express Nint.U.UAS under the control of Scer\GAL4esg.PU and are DroncKO.-FRT.QF/DroncKO transheterozygous show decreased proportion of progenitors.

      (Arthurton et al., 2020)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      DroncKO
      (Xu et al., 2022, Arthurton et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)