FlyBase Allele Report: Scer\GAL4[VP16.CNMa]

General Information

Symbol

Scer\GAL4^VP16.CNMa

Species

S. cerevisiae

Name

FlyBase ID

FBal0373753

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{CNMa-GAL4::VP16}

Also Known As

CNMa-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhang et al., 2021)

Progenitor genotype

Carried in construct

P{CNMa-GAL4::VP16}

Cytology

Description

Sequence encoding the GAL4::VP16 driver has been inserted into the CH321-34F01 BAC clone, immediately after the translation start site of CNMa. This results in expression of the GAL4::VP16 driver under the control of CNMa regulatory sequences.

(Zhang et al., 2021)

Allele components

Component

Use(s)

Regulatory region(s)

CNMa

Encoded product / tool

GAL4::VP16

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

CNMamide neuron

(Zhang et al., 2022)

Additional Information

Statement

Reference

Marker for

CNMamide neuron

(Zhang et al., 2022)

Reflects expression of

CNMa

(Zhang et al., 2022)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | oogenesis, with AstC^GD3649

(Zhang et al., 2021)

abnormal circadian rhythm | oogenesis, with Hsap\KCNJ2^UAS.EGFP

(Zhang et al., 2021)

decreased fecundity | female, with TrpA1^UAS.(B).cKa

(Zhang et al., 2021)

decreased size | adult stage, with Bhal\NaChBac^UAS.EGFP

(Zhang et al., 2021)

visible | adult stage, with Bhal\NaChBac^UAS.EGFP

(Zhang et al., 2021)

Phenotype Manifest In

egg chamber | decreased number | oogenesis, with AstC^GD3649

(Zhang et al., 2021)

egg chamber | decreased number | oogenesis, with Hsap\KCNJ2^UAS.EGFP

(Zhang et al., 2021)

wing, with Bhal\NaChBac^UAS.EGFP

(Zhang et al., 2021)

Detailed Description

Statement

Reference

Activation of Scer\GAL4^VP16.CNMa-positive neurons (by the expression of TrpA1^UAS.(B).cKa under the control of Scer\GAL4^VP16.CNMa) leads to decreased female fecundity. Activation of Scer\GAL4^VP16.CNMa-positive neurons (by the expression of Bhal\NaChBac^UAS.EGFP under the control of Scer\GAL4^VP16.CNMa) leads to small individuals, as assessed by the wing size.

Inactivation of Scer\GAL4^VP16.CNMa-positive neurons (by the expression of Hsap\KCNJ2^UAS.EGFP under the control of Scer\GAL4^VP16.CNMa) leads to absent circadian vitellogenesis rhythm (i.e. absence of peak stage 8 oocyte production at CT14).

(Zhang et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Bhal\NaChBac^UAS.EGFP, Scer\GAL4^VP16.CNMa has decreased size | adult stage phenotype, suppressible by AstC¹/AstC¹

(Zhang et al., 2021)

Bhal\NaChBac^UAS.EGFP, Scer\GAL4^VP16.CNMa has visible | adult stage phenotype, suppressible by AstC¹/AstC¹