A TI{CRIMIC.TG4.1} DNA cassette has been inserted into gpp, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequence used to target the gene was: TAAAAGAGGCCCCTCCTATACGG.
Hsap\DOT1LC45G.UAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Hsap\DOT1LE123K.UAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Scer\GAL4gpp-CR70526-TG4.1/Hsap\DOT1LL626V.UAS.Tag:HA is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Hsap\DOT1LUAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Hsap\DOT1LK1025M.UAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Hsap\DOT1LE129K.UAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Scer\GAL4gpp-CR70526-TG4.1/Hsap\DOT1LR292C.UAS.Tag:HA is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193
Hsap\DOT1LT100M.UAS.Tag:HA/Scer\GAL4gpp-CR70526-TG4.1 is a non-suppressor of lethal - all die during larval stage phenotype of gppCR70526-TG4.1/Df(3R)BSC193