FlyBase Allele Report: Dmel\PDZ-GEF[CR71002-KO-kG4]

General Information

Symbol

Dmel\PDZ-GEF^{CR71002-KO-kG4}

Species

D. melanogaster

Name

FlyBase ID

FBal0392705

Feature type

allele

Associated gene

Dmel\PDZ-GEF

Associated Insertion(s)

TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}

Cytology

Description

A TI{KozakGAL4} DNA cassette has been inserted into PDZ-GEF, replacing the coding sequence (coordinates of deleted sequence are 2L:6325265..6331096 , release 6 genome). This results in a simultaneous knock-out of PDZ-GEF plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of PDZ-GEF (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ACAGCAAAAGCTACCCCTCATGG and GATACGTTGTTTCTGATTGCTGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{KozakGAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion_site

2L:6,325,265..6,331,096 [+]

(Bereshneh et al., 2026, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

abnormal neuroanatomy | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

decreased rate of larval locomotory behavior (with Df(2L)BSC186)

(Bereshneh et al., 2026)

lethal (with Df(2L)BSC186)

(Bereshneh et al., 2026)

partially lethal

(Bereshneh et al., 2026)

some die during P-stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

microtubule | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

motor neuron | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

NMJ bouton | increased number | larval stage (with Df(2L)BSC186)

(Bereshneh et al., 2026)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has lethal phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has decreased rate of larval locomotory behavior phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal behavior | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has some die during P-stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

NOT suppressed by

Statement

Reference

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has lethal phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has lethal phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has lethal phenotype, non-suppressible by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has lethal phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has decreased rate of larval locomotory behavior phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal behavior | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has decreased rate of larval locomotory behavior phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has decreased rate of larval locomotory behavior phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has decreased rate of larval locomotory behavior phenotype, non-suppressible by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal behavior | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal behavior | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal behavior | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

Phenotype Manifest In

Suppressed by

Statement

Reference

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has microtubule | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has motor neuron | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has microtubule | larval stage phenotype, suppressible by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has motor neuron | larval stage phenotype, suppressible | partially by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has NMJ bouton | increased number | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

NOT suppressed by

Statement

Reference

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has microtubule | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has motor neuron | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has microtubule | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has microtubule | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has motor neuron | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has motor neuron | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS

(Bereshneh et al., 2026)

PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186 has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

(Bereshneh et al., 2026)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600126

y¹ w^*; TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

PDZ-GEF^{CR71002-KO-kG4}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close