FlyBase Allele Report: Scer\GAL4[PDZ-GEF-CR71002-KO-kG4]

General Information

Symbol

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

Species

S. cerevisiae

Name

FlyBase ID

FBal0392755

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}

Carried in Construct

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}

Cytology

Description

A TI{KozakGAL4} DNA cassette has been inserted into PDZ-GEF, replacing the coding sequence (coordinates of deleted sequence are 2L:6325265..6331096 , release 6 genome). This results in a simultaneous knock-out of PDZ-GEF plus a knock-in of GAL4 that is expected to be expressed under the control of the endogenous regulatory sequences of PDZ-GEF (predicted to gene trap all annotated transcripts of the gene). The TI{KozakGAL4} cassette was inserted via the CRISPR/Cas-9 drop-in technique, using a dsDNA donor vector with homology arms of length 200bp. The sgRNA sequences used to target the gene were: ACAGCAAAAGCTACCCCTCATGG and GATACGTTGTTTCTGATTGCTGG.

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{KozakGAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion_site

2L:6,325,265..6,331,096 [+]

(Bereshneh et al., 2026, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of lethal phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of decreased rate of larval locomotory behavior phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of abnormal behavior | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of some die during P-stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of abnormal neuroanatomy | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of lethal phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of lethal phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of lethal phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of lethal phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of decreased rate of larval locomotory behavior phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of abnormal behavior | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of decreased rate of larval locomotory behavior phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of decreased rate of larval locomotory behavior phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of decreased rate of larval locomotory behavior phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of abnormal behavior | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of abnormal behavior | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of abnormal behavior | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of microtubule | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of motor neuron | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of microtubule | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor | partially of motor neuron | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of NMJ bouton | increased number | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^UAS.cBa/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of microtubule | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of motor neuron | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^V152R.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of NMJ bouton | increased number | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of microtubule | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of microtubule | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of motor neuron | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of motor neuron | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of NMJ bouton | increased number | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^M253T.UAS is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}/Hsap\RAPGEF2^R145G.UAS is a non-suppressor of NMJ bouton | increased number | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Hsap\RAPGEF2^R1212X.UAS/Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4} is a non-suppressor of NMJ bouton | increased number | larval stage phenotype of PDZ-GEF^{CR71002-KO-kG4}/Df(2L)BSC186

(Bereshneh et al., 2026)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600126

y¹ w^*; TI{KozakGAL4}PDZ-GEF^{CR71002-KO-kG4}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^{PDZ-GEF-CR71002-KO-kG4}

Name Synonyms

Secondary FlyBase IDs

References (2)

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