FlyBase Allele Report: Dmel\baz[S180D.UASz.Tag:polyHis,EGFP]

General Information

Symbol

Dmel\baz^{S180D.UASz.Tag:polyHis,EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0404912

Feature type

allele

Associated gene

Dmel\baz

Associated Insertion(s)

Carried in Construct

P{UASz-baz.S180D.EGFP}

Key Links

Transgenic product class

missense_variant

(Loyer et al., 2024)

polypeptide_post_translational_processing_variant

(Loyer et al., 2024)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Loyer et al., 2024)

polypeptide_post_translational_processing_variant

(Loyer et al., 2024)

Mutagen

in vitro construct

(Loyer et al., 2024)

Progenitor genotype

Carried in construct

P{UASz-baz.S180D.EGFP}

Cytology

Description

UASz sequences regulate expression of the baz-PA isoform, mutated to carry a S180D amino acid substitution (phosphomimetic mutation), and tagged between residues K40 and P41 with Tag:polyHis and EGFP.

(Loyer et al., 2024)

Allele components

Component

Use(s)

Regulatory region(s)

UASz

binary expression system - regulatory region

Encoded product / tool

baz

Tagged with

EGFP

green fluorescent protein

Tag:polyHis

epitope tag

purification tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | larval stage, with Disc\RFP^HMS05713, Scer\GAL4^neur-P72, baz^mScarlet

(Loyer et al., 2024)

Phenotype Manifest In

basal part of cell | larval stage, with Disc\RFP^HMS05713, Scer\GAL4^neur-P72, baz^mScarlet

(Loyer et al., 2024)

larval neuroblast, with Disc\RFP^HMS05713, Scer\GAL4^neur-P72, baz^mScarlet

(Loyer et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

baz^{S180D.UASz.Tag:polyHis,EGFP}, Scer\GAL4^pnr-MD237 is a suppressor | partially of visible | adult stage phenotype of Delta^JF02867, Disc\RFP^HMS05713, Scer\GAL4^pnr-MD237, baz^mScarlet

(Loyer et al., 2024)

Other

Statement

Reference

Delta^JF02867, Disc\RFP^HMS05713, Scer\GAL4^pnr-MD237, baz^{S180D.UASz.Tag:polyHis,EGFP}, baz^mScarlet has visible | adult stage phenotype

(Loyer et al., 2024)

Delta^JF02867, Disc\RFP^HMS05713, Scer\GAL4^pnr-MD237, baz^{S180D.UASz.Tag:polyHis,EGFP}, baz^mScarlet has abnormal cell number | P-stage phenotype

(Loyer et al., 2024)

Phenotype Manifest In

Suppressor of

Statement

Reference

baz^{S180D.UASz.Tag:polyHis,EGFP}, Scer\GAL4^pnr-MD237 is a suppressor | partially of notum phenotype of Delta^JF02867, Disc\RFP^HMS05713, Scer\GAL4^pnr-MD237, baz^mScarlet

(Loyer et al., 2024)

NOT Suppressor of

Statement

Reference

baz^{S180D.UASz.Tag:polyHis,EGFP}, Scer\GAL4^pnr-MD237 is a non-suppressor of microchaeta | decreased number phenotype of Delta^JF02867, Disc\RFP^HMS05713, Scer\GAL4^pnr-MD237, baz^mScarlet

(Loyer et al., 2024)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

baz^{S180D.UASz.Tag:polyHis,EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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