FlyBase Allele Report: Dmel\Raf[CA.UAS.cUa]

General Information

Symbol

Dmel\Raf^CA.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407468

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{UAS-Raf.CA.U}

Also Known As

UAS-Raf^GOF, Raf^Act

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Raf.CA.U}

Cytology

Description

UAS regulatory sequences drive expression of a consitutively active form of Raf.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of high grade glioma

CEA

(Xie et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of high grade glioma

is ameliorated by slo^JF02146

(Xie et al., 2023)

is ameliorated by slo^KO

(Xie et al., 2023)

is exacerbated by slo^UAS.cXa

(Xie et al., 2023)

is ameliorated by slo^HMC04093

(Xie et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

visible | homeotic, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

Phenotype Manifest In

adult heart, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

adult heart muscle cell, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

antenna | ectopic, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

cardial cell, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

enterocyte, with Scer\GAL4^NP7397

(Jiang et al., 2011)

eye, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

eye disc morphogenetic furrow | ectopic, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

intestinal stem cell, with Scer\GAL4^NP7397

(Jiang et al., 2011)

myofilament, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

nuclear chromosome, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

wing vein | ectopic, with Scer\GAL4^CY2

(Guichard et al., 2006)

Detailed Description

Statement

Reference

Hearts from flies expressing Scer\GAL4^tin.CΔ4>phl^Act.Scer\UAS show reduced end-diastolic dimensions relative to controls. The transgenic hearts display myofiber disarray and increased heart wall thickness compared with controls. The number of cardiomyocytes is similar in the hearts from the transgenic flies to that of wild-type. Compared with controls, the transgenic hearts show an increase in cardiomyocyte ploidy, suggesting abnormal endoreplication.

(Yu et al., 2013)

Expression of phl^Act.Scer\UAS in the midgut, under the control of Scer\GAL4^esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

(Jiang et al., 2011)

Expression of phl^Act.Scer\UAS under the control of Scer\GAL4^CY2 results in a strong ectopic wing vein phenotype.

(Guichard et al., 2006)

Expression of phl^Act.Scer\UAS under the control of Scer\GAL4^ey.PB can result in homeotic transformation of the eye to antenna. The ectopic antenna has all segments including the arista and is oriented similarly to the endogenous antenna.

(Kumar and Moses, 2001)

Expression of phl^Act.Scer\UAS under the control of Scer\GAL4^dpp.blk1 results in ectopic furrow formation in the eye disc.

(Kumar and Moses, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Raf^CA.UAS.cUa, Scer\GAL4^tin.CΔ4 has abnormal endomitotic cell cycle phenotype, suppressible by fzr^GD9960, Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Raf^CA.UAS.cUa, Scer\GAL4^tin.CΔ4 has cardial cell phenotype, suppressible by fzr^GD9960, Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

Raf^CA.UAS.cUa has wing vein | ectopic phenotype, suppressible by Scer\GAL4^CY2/Bant\lef^UAS.cGa

(Guichard et al., 2006)

NOT suppressed by

Statement

Reference

Raf^CA.UAS.cUa, Scer\GAL4^dpp.blk1 has eye disc morphogenetic furrow | ectopic phenotype, non-suppressible by wg^UAS.cLa, Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The cardiac specific expression of rap^GD9960 under the control of Scer\GAL4^tin.CΔ4 prevents the phl^Act.Scer\UAS-induced increases in cardiomyocyte ploidy.

(Yu et al., 2013)

Furrow initiation is blocked in eye discs coexpressing phl^Act.Scer\UAS and N^DN.Scer\UAS under the control of Scer\GAL4^dpp.blk1.

(Kumar and Moses, 2001)

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\lef^Scer\UAS.cGa suppresses the strong ectopic vein phenotype of phl^Act.Scer\UAS under the control of Scer\GAL4^CY2.

(Guichard et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Raf^Act.Scer\UAS

Raf^Act.UAS

Raf^CA.UAS.cUa

phl^Act.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

FBal0359609
FBal0124968

References (16)

Report Sections

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