A T2A-GAL4-3xSTOP sequence cassette has been inserted 1 codon upstream of the splice site in the extended exon 3 of the annotated ovo-RA transcript. In this design, translation begins at the endogenous ovo-B start codon, and the presence of the T2A sequence ensures separate translation of the GAL4 driver.
ovoB.ext-.UASp.3xFHA/Scer\GAL4ovo-ovo-GAL4 fails to rescue ovoovo-GAL4/ovoΔBP