The GMR71G01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
The GMR22G11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
In a split-GAL4 system, HsapRELAAD.R71G01 drives expression in 10-13 adult fruitless pMP-e (male) neurons and 0-4 neurons in the lateral horn of male brains when combined with ScerGAL4DBD.R22G11.
In a split-GAL4 system, ScerGAL4DBD.R22G11 drives expression in 8-10 fruitless pMP-e (male) neurons when combined with HsapRELAAD.R15A01 and 10-13 of these neurons when combined with HsapRELAAD.R71G01, 0-4 neurons in the lateral horn of male brains are labelled in both combinations.
Labels 10-13 fruitless pMP-e (male) neurons and 0-4 neurons in the lateral horn of male brains.
Labels 10-13 adult fruitless pMP-e (male) neurons and 0-4 neurons in the lateral horn of male brains.