FlyBase Allele Report: Scer\GAL4[DBD.R22G11]

General Information

Symbol

Scer\GAL4^DBD.R22G11

Species

S. cerevisiae

Name

FlyBase ID

FBal0331499

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R22G11-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R22G11-GAL4.DBD}

Cytology

Description

The GMR22G11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR22G11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

adult fruitless P1 (male) neuron | subset

Comment: when combined with P{R15A01-p65.AD}

when combined with P{R71G01-p65.AD}

(Hoopfer et al., 2015)

adult lateral horn | restricted

Comment: when combined with P{R15A01-p65.AD}

when combined with P{R71G01-p65.AD}

(Hoopfer et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 29[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R22G11 and Hsap\RELA^AD.R15A01, Scer\GAL4^DBD.R22G11 and Hsap\RELA^AD.R57C10, or Scer\GAL4^DBD.R22G11 and Hsap\RELA^AD.R71G01, in adult males significantly increases aggressive lunging behavior and wing extensions towards other males, compared to controls.

(Hoopfer et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

69828

w¹¹¹⁸; P{R22G11-GAL4.DBD}attP2

87614

w¹¹¹⁸; P{R20G06-p65.AD}attP40/CyO, P{2xTb¹-RFP}CyO; P{R22G11-GAL4.DBD}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R22G11.T:Zzzz\Zip-}

Scer\GAL4^DBD.R22G11

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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