FlyBase Recombinant Construct Report: P{R15A01-p65.AD}

General Information

Symbol

P{R15A01-p65.AD}

FlyBase ID

FBtp0112075

Feature type

transgenic_transposable_element

Component Allele(s)

Hsap\RELA^AD.R15A01

Also Known As

R15A01-AD, R15A01-p65.AD, P1-Gal4 AD, R15A01-p65ADZp

Size

Expression Data

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component allele Hsap\RELA^AD.R15A01

Transgenic product class

Component

Use(s)

Regulatory region(s)

GMR15A01

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The GMR15A01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Hsap\RELA^AD.R15A01

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

adult fruitless P1 (male) neuron | subset

Comment: when combined with P{R22G11-GAL4.DBD}

when combined with P{R71G01-GAL4.DBD}

(Hoopfer et al., 2015)

adult lateral horn | restricted

Comment: when combined with P{R22G11-GAL4.DBD}

when combined with P{R71G01-GAL4.DBD}

(Hoopfer et al., 2015)

adult stage | female

adult lateral horn | restricted

Comment: when combined with P{R71G01-GAL4.DBD}

(Hoopfer et al., 2015)

adult stage

adult fruitless P1 (male) neuron | subset

Comment: when combined with P{R71G01-GAL4.DBD} (combination referred to as 'P1-splitGAL4')

when combined with P{R22D03-GAL4.DBD} (combination referred to as 'P1b')

when combined with P{R71G01-GAL4.DBD} (combination referred to as 'P1a')

(Wu et al., 2019, Zhang et al., 2018)

adult fruitless P1 (male) neuron

Comment: when combined with P{R21G01-GAL4.DBD} (combination referred to as 'P1a')

(Jung et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (3)

Stocks containing insertion(s) of this recombinant construct

Bloomington

68837

w¹¹¹⁸; P{R15A01-p65.AD}attP40

600660

w¹¹¹⁸; P{R15A01-p65.AD}JK73A/TM3, Sb¹

88313

w¹¹¹⁸; P{R15A01-p65.AD}attP40; P{VT044854-GAL4.DBD}attP2/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

15A01-AD

(Jung et al., 2020)

15A01-p65.AD

(Hindmarsh Sten et al., 2025)

P1-GAL4

(Inagaki et al., 2014)

P1-Gal4 AD

(Grover et al., 2016, Clowney et al., 2015)

P{R15A01-GAL4.AD}

(FlyBase, 1992-)

P{R15A01-p65.AD}

(FlyBase, 1996-)

R15A01-AD

(Gao et al., 2025, Zhao et al., 2024, Shen et al., 2023, Han et al., 2022, Chen et al., 2021, Agrawal and Dickinson, 2019, Liu et al., 2019, Zhang et al., 2018, Watanabe et al., 2017, Hoopfer et al., 2015, Kallman et al., 2015)

R15A01-p65.AD

(Steinfath et al., 2025, Watanabe et al., 2024, Wu et al., 2020)

R15A01-p65AD:Zp

(Ishii et al., 2020)

R15A01-p65ADZp

(Bonheur et al., 2023, Watanabe et al., 2017)

R15A01.AD

(Wu et al., 2020)

Secondary FlyBase IDs

References (31)

Report Sections

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