E1 enzymes use ATP to activate the carboxyl terminus of ubiquitin or ubiquitin-like proteins forming a high-energy AMP conjugate, which is then subsequently transferred to the E1 active site cysteine to form a thioester intermediate. In many cases, the ubiquitin/ubiquitin-like molecule is then transferred to an E2 active site cysteine via trans-thioesterification. (Adapted from PMID:23800009).
Notes on Group
Source Material
The
E1 UBIQUITIN-LIKE ACTIVATING ENZYMES
Gene Group has been compiled using the following publication(s):
Du et al., 2011 and Pham and Sauer, 2000
.
