FlyBase Gene Report: Dmel\Ebp

General Information

Symbol

Dmel\Ebp

Species

D. melanogaster

Name

Ejaculatory bulb protein

Annotation Symbol

CG2668

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004181

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Ejaculatory bulb protein (Ebp) encodes a 377-amino acid protein produced in the male ejaculatory bulb. It is essential for the formation and integrity of the posterior mating plug formed in females after mating. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

PEB-me, Peb, Protein ejaculatory bulb

Key Links

Genomic Location

Cytogenetic map

60F5-60F5

Sequence location

2R:25,175,126..25,176,715 [-]

Recombination map

2-107

RefSeq locus

NT_033778 REGION:25175126..25176715

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in insemination

inferred from high throughput expression pattern

(Findlay et al., 2009, Findlay et al., 2008)

involved_in mating plug formation

inferred from expression pattern

(Lung and Wolfner, 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mating plug formation

traceable author statement

(Chapman, 2001)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Lung and Wolfner, 2001)

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Major protein component of the posterior mating plug. Accessory gland proteins constitute, or are required for formation of the anterior mating plug. Posterior mating plug forms before sperm transfer and the anterior mating plug is formed after the start of mating.

(UniProt, Q9U6L5)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ebp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9U6L5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ebp-RA

FBtr0072454

NM_138151

1202

377

Ebp-RB

FBtr0333174

NM_001274249

1529

377

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ebp-PA

FBpp0072356

37.8

377

10.08

FBpp0305377

37.8

377

10.08

Q9U6L5

NP_001261178

AGB93708

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

377 aa isoforms: Ebp-PA, Ebp-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ebp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Peb_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

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Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Lung and Wolfner, 2001)

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2009, Findlay et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ebp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ebp

View exonic expression by tissue for Dmel\Ebp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004181

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38009/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004181

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004181

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004181

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2668

Flygut - An atlas of the Drosophila adult midgut

FBgn0004181

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ebp

Transgenic constructs containing regulatory region of Ebp

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(2R)Kr10

(Ludwig et al., 1991)

Ts(1Lt;2Lt)sc^S2

(Ludwig et al., 1991)

Gene not disrupted in

Df(2R)Kr1

(Ludwig et al., 1991)

Df(2R)M60E

(Ludwig et al., 1991)

Df(2R)Px1

(Ludwig et al., 1991)

Df(2R)Px2

(Ludwig et al., 1991)

In(2LR)Px⁴

(Ludwig et al., 1991)

Ts(Y;2Lt)B80

(Ludwig et al., 1991)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ebp^GD5852

viable, with Scer\GAL4^Mef2.PR

Ebp^GD5852

viable, with Scer\GAL4^pnr-MD237

Ebp^GD5852

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\PCLO

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\NUP159

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ebp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ebp - gNacα

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63576

DroID - A comprehensive database of gene and protein interactions.

FBgn0004181

MIST (protein-protein) - An integrated Molecular Interaction Database

38009

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-107

Cytogenetic map

60F5-60F5

Sequence location

2R:25,175,126..25,176,715 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60F5-60F5

Limits computationally determined from genome sequence between P{EP}CG2790^EP412&P{EP}CG3776^EP835 and P{EP}zip^EP856&P{PZ}l(2)10481⁰³²⁶³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60E11-60F5

(Uspenskii et al., 1990)

Experimentally Determined Recombination Data

Location

2-107

(FlyBase, 2016)

2-111.8

(Comeron et al., 2012)

2-107.3--2-108

(Ludwig et al., 1991)

2-107.2--108.0

(Uspenskii et al., 1990)

Left of (cM)

Right of (cM)

βTub60D

(Ludwig et al., 1991)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

58522

y¹ w^*; Mi{MIC}Ebp^MI12466

55390

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04078}attP40

VDRC

v100183

P{KK104312}VIE-260B

v18973

w¹¹¹⁸; P{GD5852}v18973

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH06048

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004181

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004181

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Peb protein is a major constituent of the posterior region of the mating plug.

(Lung and Wolfner, 2001)

The genetic control and expression of Peb has been studied.

(Ludwig et al., 1991)

The major protein of the ejaculatory bulb of D.melanogaster is made up of 4-5 subfractions. These are encoded by a single locus (Peb).

(Uspenskii et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: Peb BcDNA:GH06048

(Lung and Wolfner, 2001)

Source for merge of: Peb anon-WO0140519.140

(FlyBase, 1996-)

Additional comments

Source for merge of Peb anon-WO0140519.140 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: BcDNA:GH06048 CG2668

(Zhong, 1999.11)

Source for identity of: Ebp Peb

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'Peb' to 'Ebp' to minimise confusion with the 'peb' ('pebbled') gene symbol, from which it differed only by case.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

BcDNA:GH06048

CG2668

(He et al., 2024, Yuan et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Metta et al., 2006, Artero et al., 2003, Whitfield, 2000.5.17)

EBP

(Dobens et al., 2021)

Ebp

(Akagi et al., 2025, Jean-François et al., 2025, Ryvkin et al., 2024, Candia et al., 2023, McDonough-Goldstein et al., 2022, Ghosh et al., 2020)

P38

(Lung and Wolfner, 2001)

PEB

(Lopez et al., 2001, Lyudvig et al., 1990, Uspenskii et al., 1990)

PEB-me

(Misra et al., 2014, Schnakenberg et al., 2012, Sun et al., 2011, Qazi et al., 2003, Chapman, 2001, Lung and Wolfner, 2001, Ludwig et al., 1991)

PEBme

(Avila et al., 2015)

Peb

(Findlay et al., 2009, Takemori and Yamamoto, 2009, Findlay et al., 2008)

anon-WO0140519.140

peb

(Uspenskii et al., 1990)

Name Synonyms

Ejaculatory bulb protein

(Garlovsky et al., 2022)

Protein ejaculatory bulb

(Cammarato et al., 2011, Takemori and Yamamoto, 2009)

ejaculatory bulb protein

(Sun et al., 2011)

Secondary FlyBase IDs

FBgn0028493
FBgn0044740

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38009

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9U6L5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9U6L5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KGF3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9U6L5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004181

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38009/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004181

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004181

FlyMine - An integrated database for Drosophila genomics

FBgn0004181

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2668

MARRVEL_MODEL - MARRVEL (model organism gene)

38009

Linkouts

BioGRID - A database of protein and genetic interactions.

63576

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004181

DroID - A comprehensive database of gene and protein interactions.

FBgn0004181

DRSC - Results frm RNAi screens

FBgn0004181

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Peb_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004181

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004181

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2668

Flygut - An atlas of the Drosophila adult midgut

FBgn0004181

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012356

MIST (protein-protein) - An integrated Molecular Interaction Database

38009

References (61)

The following external sites may use different assemblies or annotations than FlyBase.

0.05

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