FlyBase Gene Report: Dmel\mex1

General Information

Symbol

Dmel\mex1

Species

D. melanogaster

Name

midgut expression 1

Annotation Symbol

CG7936

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004228

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Involved in morphogenesis and development. (UniProt, P23487)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

71D4-71D4

Sequence location

3L:15,517,424..15,519,592 [-]

Recombination map

3-43

RefSeq locus

NT_037436 REGION:15517424..15519592

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Involved in morphogenesis and development.

(UniProt, P23487)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mex1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P23487)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0075602

710

FBtr0303786

741

FBtr0334084

1034

Additional Transcript Data and Comments

Reported size (kB)

0.95 (northern blot)

(Schulz et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mex1-PA

FBpp0075355

9.4

8.25

FBpp0292798

9.4

8.25

FBpp0306209

9.4

8.25

P23487

NP_001261880

AGB94573

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

83 aa isoforms: mex1-PA, mex1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

83 (aa)

(Schulz et al., 1991)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mex1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.29

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 17

posterior midgut primordium

(Schulz et al., 1991)

anterior midgut primordium

(Schulz et al., 1991)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

gastric caecum

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

(Schulz et al., 1991)

embryonic stage 12

(Schulz et al., 1991)

embryonic stage 14 -- 17

embryonic/larval midgut

(Schulz et al., 1991)

adult stage

(Schulz et al., 1991)

Additional Descriptive Data

mex1 transcripts are detected in mid and late embryos and in adults on northern blots. They are detected by in situ hybridization in the anterior and posterior midgut primordia starting in stage 12.

(Schulz et al., 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{mex1-GAL4.2.1}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval midgut

(Sieber and Thummel, 2012, Tennessen et al., 2011, Mazock et al., 2010)

larval midgut enterocyte

(Redhai et al., 2020)

third instar larval stage

embryonic/larval midgut

(Binks et al., 2010)

adult stage

adult midgut

(Palu and Thummel, 2016, Chen et al., 2014)

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mex1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mex1

View exonic expression by tissue for Dmel\mex1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004228

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39677/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004228

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004228

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7936

Flygut - An atlas of the Drosophila adult midgut

FBgn0004228

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\mex1

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mex1

Transgenic constructs containing regulatory region of mex1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

mex1^c04603

viable, with Scer\GAL4^pnr-MD237

mex1^GD1662

Sterility

Allele

fertile

mex1^c04603

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

mex1^GD1662

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP012396

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mex1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG42394

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mex1 - CNT2

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64997

DroID - A comprehensive database of gene and protein interactions.

FBgn0004228

MIST (protein-protein) - An integrated Molecular Interaction Database

39677

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-43

Cytogenetic map

71D4-71D4

Sequence location

3L:15,517,424..15,519,592 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

71D4-71D4

Limits computationally determined from genome sequence between P{lacW}cp309^s2172&P{lacW}l(3)j2A2^j2A2 and P{EP}Tfb2^EP572

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-43

(FlyBase, 2016)

3-37.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

91368

w¹¹¹⁸; P{mex1-GAL4.2.1}10-8

11532

w¹¹¹⁸; PBac{PB}mex1^c04603

91367

P{mex1-GAL4.2.1}9-1, y¹ w¹¹¹⁸

53861

y¹ w^*; Mi{MIC}mex1^MI10488

56607

y¹ w^*; Mi{MIC}mex1^MI11421

605142

y^* w^*; TI{2A-QF2}mex1^2A-QF2

604843

y^* w^*; TI{2A-lexA::GAD}mex1^2A-LG

91369

w¹¹¹⁸; TM2, P{mex1-GAL4.2.1}12-8/TM6B, Tb¹

VDRC

v100456

P{KK104392}VIE-260B

v11809

w¹¹¹⁸; P{GD1662}v11809/CyO

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE66244

Other clones

MIP07124

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004228

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004228

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

RNase protection studies show that the 3' end of mex1 is located approximately 4kb downstream of Eip71CD, with the two genes convergently transcribed.

(Schulz et al., 1991)

mex1 has been sequenced and its RNA expression pattern analysed.

(Schulz et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG7936

(Keleman et al., 2009.8.5)

Mex1

(Ewen-Campen et al., 2026)

mex1

(Hu et al., 2017.6.13, Okumura et al., 2007, Choksi et al., 2006, Phillips and Thomas, 2006)

Name Synonyms

MEX

(Binks et al., 2010)

Midgut expression 1

(Cho et al., 2005)

midgut expression 1

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39677

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P23487

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P23487

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P23487

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004228

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39677/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004228

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004228

FlyMine - An integrated database for Drosophila genomics

FBgn0004228

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7936

MARRVEL_MODEL - MARRVEL (model organism gene)

39677

Linkouts

BioGRID - A database of protein and genetic interactions.

64997

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004228

DroID - A comprehensive database of gene and protein interactions.

FBgn0004228

DRSC - Results frm RNAi screens

FBgn0004228

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004228

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004228

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7936

Flygut - An atlas of the Drosophila adult midgut

FBgn0004228

MIST (protein-protein) - An integrated Molecular Interaction Database

39677

References (48)

The following external sites may use different assemblies or annotations than FlyBase.

-0.29

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