FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Gene: Dmel\Tim8
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General Information
Symbol
Dmel\Tim8
Species
D. melanogaster
Name
Tim8
Annotation Symbol
CG1728
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027359
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The Tim8-Tim13 complex mediates the import of some proteins while the predominant Tim9-Tim10 70 kDa complex mediates the import of much more proteins (By similarity). (UniProt, Q9Y1A3)
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
10B6-10B6
Sequence location
Recombination map
1-34
RefSeq locus
NC_004354 REGION:11368445..11369283
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables unfolded protein holdase activity
inferred from sequence or structural similarity with UniProtKB:P57744
(Gene Ontology Curators, 2002-)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein insertion into mitochondrial inner membrane
inferred from sequence or structural similarity with UniProtKB:O60220
(Gene Ontology Curators, 2002-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of mitochondrial intermembrane space chaperone complex
inferred from sequence or structural similarity with UniProtKB:O60220
(Gene Ontology Curators, 2002-)
located_in mitochondrial intermembrane space
inferred from sequence or structural similarity with UniProtKB:O60220
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the small Tim family. (Q9Y1A3)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
TIM8-TIM13 COMPLEX - CG34132 VARIANT -
The Tim8-Tim13 complex is a mitochondrial intermembrane space chaperone that is required for the import and insertion of a subset of multipass transmembrane proteins into the mitochondrial inner membrane. (Adapted from PMID:11875042 and PMID:18706423.)
TIM8-TIM13 COMPLEX - TIM13 VARIANT (TESTIS) -
The Tim8-Tim13 complex is a mitochondrial intermembrane space chaperone that is required for the import and insertion of a subset of multipass transmembrane proteins into the mitochondrial inner membrane. (Adapted from PMID:11875042 and PMID:18706423.)
SMALL TIMS -
The small TIMs are a family of mitochondrial intermembrane space holdases that facilitate the transfer of membrane proteins imported across the outer mitochondrial membrane by the TOM complex and destined for insertion into the outer or inner membrane. Acting as heteromers, they prevent the aggregation of the hydrophobic segments membrane proteins and transferring them to the appropriate insertase or translocase complex. (Adapted from PMID:19585663.)
Protein Function (UniProtKB)
Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The Tim8-Tim13 complex mediates the import of some proteins while the predominant Tim9-Tim10 70 kDa complex mediates the import of much more proteins (By similarity).
(UniProt, Q9Y1A3)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Tim8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9Y1A3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.46

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Tim8-RA
FBtr0073482
NM_132485
661
88
Tim8-RB
FBtr0343640
NM_001298191
761
88
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Tim8-PA
FBpp0073338
10.1
88
4.80
Q9Y1A3
NP_572713
AAF48036
Tim8-PB
FBpp0310233
10.1
88
4.80
Q9Y1A3
NP_001285120
AHN59590
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

88 aa isoforms: Tim8-PA, Tim8-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of Tim8 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).

(UniProt, Q9Y1A3)
Subunit Structure (UniProtKB)

Heterohexamer; composed of 3 copies of Tim8 and 3 copies of Tim13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of Tim22 (By similarity).

(UniProt, Q9Y1A3)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tim8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.22

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tim8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Tim8
Transgenic constructs containing regulatory region of Tim8
Aberrations (Deficiencies and Duplications) ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\TIMM8B
12 of 14
Yes
Yes
1  
Hsap\TIMM8A
7 of 14
No
Yes
1  
Hsap\TIMM9
1 of 14
No
No
1  
Hsap\TIMM10B
1 of 14
No
No
1  
Hsap\TIMM10
1 of 14
No
No
1  
Hsap\TIMM13
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (8)
Rnor\Timm8b
13 of 14
Yes
Yes
Rnor\Timm8a2
7 of 14
No
Yes
Rnor\Timm8a1
6 of 14
No
Yes
Rnor\LOC689395
5 of 14
No
Yes
Rnor\Timm9
1 of 14
No
No
Rnor\Timm10b
1 of 14
No
No
Rnor\Timm10
1 of 14
No
No
Rnor\Timm13
1 of 14
No
No
Mus musculus (laboratory mouse) (7)
Mmus\Timm8b
12 of 14
Yes
Yes
Mmus\Timm8a1
7 of 14
No
Yes
Mmus\Timm8a2
7 of 14
No
Yes
Mmus\Timm9
1 of 14
No
No
Mmus\Timm10b
1 of 14
No
No
Mmus\Timm10
1 of 14
No
No
Mmus\Timm13
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\timm8b
10 of 13
Yes
Yes
Xtro\timm8a
1 of 13
No
Yes
Xtro\timm10
1 of 13
No
No
Danio rerio (Zebrafish) (5)
Drer\timm8b
13 of 14
Yes
Yes
Drer\timm8a
7 of 14
No
Yes
Drer\timm10b
1 of 14
No
No
Drer\timm10
1 of 14
No
No
Drer\timm13
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\ddp-1
9 of 14
Yes
Yes
Cele\tin-9.1
1 of 14
No
No
Cele\tin-10
1 of 14
No
No
Cele\tin-13
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (5)
Agam\AgaP_AGAP010606
12 of 12
Yes
Yes
Agam\AgaP_AGAP000781
1 of 12
No
No
Agam\AgaP_AGAP002277
1 of 12
No
No
Agam\AgaP_AGAP007167
1 of 12
No
No
Agam\AgaP_AGAP010679
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (4)
Atha\TIM8
11 of 13
Yes
Yes
Atha\TIM9
1 of 13
No
No
Atha\TIM10
1 of 13
No
No
Atha\TIM13
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\TIM8
11 of 13
Yes
Yes
Scer\ALR1
1 of 13
No
Yes
Scer\ALR2
1 of 13
No
Yes
Scer\TIM9
1 of 13
No
No
Scer\TIM10
1 of 13
No
No
Scer\TIM13
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\tim8
11 of 12
Yes
Yes
Spom\tim9
1 of 12
No
No
Spom\tim10
1 of 12
No
No
Spom\tim13
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Tim8. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (5)
CG34132
2 of 13
CG42302
2 of 13
Tim13
2 of 13
Tim9a
1 of 13
Tim10
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TIMM8B; translocase of inner mitochondrial membrane 8 homolog B
    12 of 14
    606659
         
        TIMM8A; translocase of inner mitochondrial membrane 8A
        7 of 14
        300356
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Pink1
        enhanceable
        Tim8
        (Gehrke et al., 2015)
        External Data
        Subunit Structure (UniProtKB)
        Heterohexamer; composed of 3 copies of Tim8 and 3 copies of Tim13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of Tim22 (By similarity).
        (UniProt, Q9Y1A3 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-34
        Cytogenetic map
        10B6-10B6
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        10B6-10B6
        Limits computationally determined from genome sequence between P{EP}CG11756EP1610 and P{EP}CG32666EP1452
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        1-34

        (FlyBase, 2016)

        1-36.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (5)
        Genomic Clones (13)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (25)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: Tim8 BcDNA:SD06593

            (FlyBase, 1996-)
            Additional comments

            Source for merge of Tim8 BcDNA:SD06593 was a shared cDNA ( date:030728 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of

            Source for identity of: Tim8 CG1728

            (Zhong, 1999.11)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (3)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 37 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (45)