FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\THADA
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General Information
Symbol
Dmel\THADA
Species
D. melanogaster
Name
THADA
Annotation Symbol
CG15618
Feature Type
protein_coding_gene
FlyBase ID
FBgn0031077
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Summary
THADA (THADA) encodes a metabolic regulator that controls the balance between fat storage and heat production. It physically interacts with the ER calcium pump encoded by SERCA and regulates its activity to control metabolism. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
18F4-18F4
Sequence location
Recombination map
1-64
RefSeq locus
NC_004354 REGION:19859743..19865524
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables calcium channel inhibitor activity
inferred from direct assay
(Moraru et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables enzyme regulator activity
inferred from biological aspect of ancestor with PANTHER:PTN000373088
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in adaptive thermogenesis
inferred from mutant phenotype
(Moraru et al., 2017)
involved_in negative regulation of endoplasmic reticulum calcium ion concentration
inferred from direct assay
(Moraru et al., 2017)
acts_upstream_of negative regulation of lipid storage
inferred from mutant phenotype
(Moraru et al., 2017)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in negative regulation of endoplasmic reticulum calcium ion concentration
inferred from biological aspect of ancestor with PANTHER:PTN000373091
(GO Reference Genome Project, 2011-)
involved_in tRNA nucleoside ribose methylation
inferred from biological aspect of ancestor with PANTHER:PTN000373088
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:Q6YHU6
(Gene Ontology Curators, 2002-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
is_active_in cytoplasmic side of endoplasmic reticulum membrane
inferred from direct assay
(Moraru et al., 2017)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the THADA family. (Q9VWB9)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
THADA (THADA) encodes a metabolic regulator that controls the balance between fat storage and heat production. It physically interacts with the ER calcium pump encoded by SERCA and regulates its activity to control metabolism. [Date last reviewed: 2018-10-04]
Protein Function (UniProtKB)
Together with methyltransferase Trm7-32, methylates the 2'-O-ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs (By similarity). Plays a key role in energy homeostasis by regulating the balance between energy storage and heat production. Functions by negatively regulating Ca(2+) signaling pathways that are involved in heat production and maintaining correct lipid storage in the fat body. Regulates Ca(2+) signaling pathways by reducing the activity of the calcium-transporting ATPase SERCA possibly by promoting uncoupling of SERCA ATP hydrolysis from calcium pumping. May also function in the nervous system to control feeding behavior (PubMed:28399403).
(UniProt, Q9VWB9)
Summary (Interactive Fly)

negative regulation of lipid storage - negative regulation of endoplasmic reticulum calcium ion concentration - adaptive thermogenesis - negative regulation of calcium-transporting ATPase activity - binds the sarco/ER Ca2+ ATPase (SERCA) and acts on it as an uncoupler

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\THADA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VWB9)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.39

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Gene model reviewed during 5.53

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
THADA-RB
FBtr0289978
NM_134517
5352
1746
THADA-RC
FBtr0308650
NM_001258841
5361
137
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
THADA-PB
FBpp0288416
197.4
1746
6.83
Q9VWB9
NP_608361
AAF49027
THADA-PC
FBpp0300873
15.6
137
10.53
M9NFG3
NP_001245770
AFH07482
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with SERCA.

(UniProt, Q9VWB9)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\THADA using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.24

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
is_active_in cytoplasmic side of endoplasmic reticulum membrane
inferred from direct assay
(Moraru et al., 2017)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\THADA in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of THADA
Transgenic constructs containing regulatory region of THADA
Aberrations (Deficiencies and Duplications) ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\THADA
10 of 14
Yes
Yes
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Thada
10 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Thada
9 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\thada
8 of 13
Yes
Yes
Xtro\LOC100492758
2 of 13
No
Yes
Danio rerio (Zebrafish) (2)
Drer\thada
9 of 14
Yes
Yes
Drer\si:ch211-225b11.4
5 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\Y92H12A.5
9 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP010263
8 of 12
Yes
Yes
Agam\AgaP_AGAP012629
3 of 12
No
Yes
Agam\AgaP_AGAP010262
2 of 12
No
Yes
Agam\AgaP_AGAP012630
1 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AT3G55160
9 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\TRM732
8 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPCC1494.07
8 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:THADA. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
CG3368
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
THADAKO1
model of  obesity
CEA
(Moraru et al., 2017)
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
THADAGD9074
ameliorates  Machado-Joseph disease
modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
(Voßfeldt et al., 2012)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
THADA; THADA armadillo repeat containing
10 of 14
THADA ARMADILLO REPEAT-CONTAINING PROTEIN; THADA
       
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      THADA - SERCA
      anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot
      (Moraru et al., 2017)
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Interacts with SERCA.
      (UniProt, Q9VWB9 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map
      1-64
      Cytogenetic map
      18F4-18F4
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      18F4-18F4
      Limits computationally determined from genome sequence between P{EP}EP1116&P{EP}EP1344EP1344 and P{EP}amnEP1649&P{EP}amnEP1442
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      1-64

      (FlyBase, 2016)

      1-63.1

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (9)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (7)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments
                  Relationship to Other Genes
                  Source for database merge of
                  Additional comments
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: THADA CG15618

                  (Moraru et al., 2017)
                  Nomenclature comments
                  Etymology

                  Named 'THADA' after the human ortholog.

                  (Moraru et al., 2017)
                  Synonyms and Secondary IDs (2)
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 38 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  References (28)