FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\THADAKO1
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General Information
Symbol
Dmel\THADAKO1
Species
D. melanogaster
Name
FlyBase ID
FBal0326345
Feature type
allele
Associated gene
Dmel\THADA
Associated Insertion(s)
TI{TI}THADAKO1
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Moraru et al., 2017)
Mutagen
ends-out gene targeting
(Moraru et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}THADAKO1
Cytology
Description

A 1024nt deletion covering the 4th exon and the beginning of the 5th exon, and causing the remainder of the open reading frame to be out-of-frame.

(Moraru et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:19,863,663..19,864,688 [-]
Comment:

A 1024nt deletion covering the 4th exon and the beginning of the 5th exon, and causing the remainder of the open reading frame to be out-of-frame.

(Moraru and Teleman, 2017.7.31)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  obesity
      CEA
      (Moraru et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      THADAKO1 mutants are viable and have normal developmental patterning, timing, adult wings size, wing cell size and lifespan compared to controls but display metabolic defects, are hyperphagic and obese (measured as a fat/protein ratio), although the robustness of the increased-feeding phenotype varies considerably with genetic background; the lipid droplets in the fat body cells of THADAKO1 adults are larger and take up larger proportion of the cell area compared to controls, the flies however do not display any significant ectopic lipid deposition in guts. The adult flies survive significantly longer upon starvation compared to controls but show impaired recovery from cold-induced paralysis and generate less heat per unit of body biomass.

      (Moraru et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The obesity (measured as fat/protein ratio) of THADAKO1 mutant adults is rescued by expression of SERCAKK107371 driven by Scer\GAL4da.PU and partially so by Scer\GAL4tub.PU-controlled expression of Itp-r83AScer\UAS.cVa.

      (Moraru et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference

      The obesity (measured as fat/protein ratio) of THADAKO1 mutant adults is rescued by expression of Hsap\THADAScer\UAS.cMa driven by Scer\GAL4hs.PU.

      (Moraru et al., 2017)
      Complementation and Rescue Data
      Partially rescued by

      THADAKO1 is partially rescued by THADAUAS.cMa/Scer\GAL4elav.PU

      (Moraru et al., 2017)

      THADAKO1 is partially rescued by THADAUAS.cMa/Scer\GAL4da.PU

      (Moraru et al., 2017)

      THADAKO1 is partially rescued by THADAUAS.cMa/Scer\GAL4mir-278-Gal4KI

      (Moraru et al., 2017)

      THADAKO1 is partially rescued by THADAUAS.cMa/Scer\GAL4Adh.PF

      (Moraru et al., 2017)
      Comments

      The obesity (measured as a fat/protein ratio) as well increased food intake characteristic for THADAKO1 mutant adults are partially rescued by expression of THADAScer\UAS.cMa controlled by either Scer\GAL4elav.PU or Scer\GAL4da.PU. The increased adiposity of THADAKO1 mutants is also improved by Scer\GAL4mir-278-Gal4KI (while the increased food intake is not rescued) or Scer\GAL4Adh.PF-driven expression.

      The poor recovery from cold-induced paralysis displayed by THADAKO1 mutants is rescued by expression of THADAScer\UAS.cMa under the control of Scer\GAL4da.PU in the mutant background.

      (Moraru et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      THADAKO1
      (Moraru and Teleman, 2017.7.31, Moraru et al., 2017)
      THADAKO
      (Moraru et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)