FlyBase Gene Report: Dmel\Vha16-1

General Information

Symbol

Dmel\Vha16-1

Species

D. melanogaster

Name

Vacuolar H⁺ ATPase 16kD subunit 1

Annotation Symbol

CG3161

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262736

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

Vacuolar H[+] ATPase 16kD subunit 1 (Vha16-1) encodes the proteolipid component of V-ATPase, which is a multi-functional channel protein. It contributes to vesicle-membrane fusion, hydrophilic molecule transport and intercellular communication. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Vha16, ductin, MRE15, V-ATPase, Vacuolar H⁺ ATPase 16kD subunit

Key Links

Genomic Location

Cytogenetic map

42B2-42B2

Sequence location

2R:6,625,242..6,633,090 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6625242..6633090

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables proton transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR002379

(InterPro Project Members, 2004-)

contributes_to proton-transporting ATPase activity, rotational mechanism

inferred by curator from GO:0033181

(Allan et al., 2005)

enables proton-transporting ATPase activity, rotational mechanism

inferred from electronic annotation with InterPro:IPR000245, InterPro:IPR011555

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in vacuolar acidification

inferred from mutant phenotype

(Redhai et al., 2020)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proton transmembrane transport

inferred from electronic annotation with InterPro:IPR000245, InterPro:IPR002379, InterPro:IPR011555

(InterPro Project Members, 2004-)

inferred by curator from GO:0033181

(Allan et al., 2005)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in fusome

inferred from direct assay

(Lighthouse et al., 2008)

part_of plasma membrane proton-transporting V-type ATPase complex

inferred from mutant phenotype

(Allan et al., 2005)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000813763

(GO Reference Genome Project, 2011-)

part_of proton-transporting two-sector ATPase complex, proton-transporting domain

inferred from electronic annotation with InterPro:IPR002379

(InterPro Project Members, 2004-)

part_of proton-transporting V-type ATPase, V0 domain

inferred from electronic annotation with InterPro:IPR000245, InterPro:IPR011555

(InterPro Project Members, 2004-)

part_of vacuolar proton-transporting V-type ATPase, V0 domain

inferred from sequence or structural similarity with SGD:S000000753

(Dow, 1999)

Gene Group (FlyBase)

VACUOLAR ATPASE V0 DOMAIN SUBUNITS

Protein Family (UniProt)

Belongs to the V-ATPase proteolipid subunit family. (P23380)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

proton-transporting ATPase activity, rotational mechanism
RHEA 57721:

Summaries

Gene Snapshot

Gene Group (FlyBase)

VACUOLAR ATPASE V0 DOMAIN SUBUNITS -: The V0 domain of V-ATPases is the integral membrane domain responsible for the translocation of protons across the membrane. It is assembled from four to five different subunits (a, c, c'', d and e in D.mel) that are expressed as multiple isoforms in a tissue-specific manner. (Adapted from PMID:20450191 and FBrf0188241).

Protein Function (UniProtKB)

Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (By similarity). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32269334). In enterocytes, acts as part of a pHCl-2 sensory pathway which mediates Tor-dependent larval growth and metabolism in response to zinc availability (PubMed:32269334). Likely acts in maintaining enterocyte lysosomal acidification which consequently promotes Tor activation at the lysosome membrane (PubMed:32269334).

(UniProt, P23380)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Vha16-1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P23380)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Vha16-1-RA

FBtr0086150

NM_057453

2833

159

Vha16-1-RB

FBtr0086151

NM_165474

2798

159

Vha16-1-RC

FBtr0086152

NM_165475

2981

159

Vha16-1-RD

FBtr0086153

NM_165476

2816

159

Additional Transcript Data and Comments

Reported size (kB)

3.1 (northern blot)

(Finbow et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Vha16-1-PA

FBpp0085483

16.3

159

8.78

FBpp0085484

16.3

159

8.78

FBpp0085485

16.3

159

8.78

FBpp0085486

16.3

159

8.78

P23380

NP_724476

AAM68381

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

159 aa isoforms: Vha16-1-PA, Vha16-1-PB, Vha16-1-PC, Vha16-1-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

159 (aa)

(Finbow et al., 1994, Meagher et al., 1990)

Comments

External Data

Subunit Structure (UniProtKB)

V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (By similarity). The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H (By similarity). The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits VhaAC45 and ATP6AP2 (By similarity).

(UniProt, P23380)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P23380

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vha16-1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Vha16-1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.08

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc

(Tognon et al., 2016)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval ventral nerve cord | punctate

(Bohrmann and Bonafede, 2000)

embryonic/larval midgut primordium

• cytoplasm | apical

(Bohrmann and Bonafede, 2000)

embryonic stage -- larval stage

embryonic/larval salivary gland

• apical plasma membrane

(Bohrmann and Bonafede, 2000)

larval stage

neuropil of larval brain | punctate

(Bohrmann and Bonafede, 2000)

somatic muscle cell | punctate

(Bohrmann and Bonafede, 2000)

adult stage

adult midgut

• brush border

(Bohrmann and Bonafede, 2000)

adult gnathal ganglion | punctate

(Bohrmann and Bonafede, 2000)

indirect flight muscle cell

• cytoplasm & apical plasma membrane

(Bohrmann and Bonafede, 2000)

oogenesis & adult stage | female

follicle cell | apical | lateral

(Lautemann and Bohrmann, 2016)

polar follicle cell

(Lautemann and Bohrmann, 2016)

centripetal follicle cell

(Lautemann and Bohrmann, 2016)

nurse cell

(Lautemann and Bohrmann, 2016)

stretch follicle cell

(Lautemann and Bohrmann, 2016)

oocyte

(Lautemann and Bohrmann, 2016)

oogenesis stage S9 -- S10

main body follicle cell | subset

Comment: expression in cells on ventral side of oocyte

(Krüger and Bohrmann, 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- first instar larval stage

organism

Comment: predominantly 29 kD isoform

(Bohrmann and Bonafede, 2000)

third instar larval stage

embryonic/larval hypodermal muscle cell

Comment: predominantly 21 kD isoform

(Bohrmann and Bonafede, 2000)

larval brain

Comment: predominantly 16 kD isoform

(Bohrmann and Bonafede, 2000)

embryonic/larval salivary gland

Comment: predominantly 16, 21 kD isoform

(Bohrmann and Bonafede, 2000)

adult stage

adult head

Comment: predominantly 21 kD isoform

(Bohrmann and Bonafede, 2000)

adult thorax

Comment: predominantly 16 kD isoform

(Bohrmann and Bonafede, 2000)

oogenesis stage S1 -- S7

egg chamber

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

oogenesis stage S10

oocyte

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

nurse cell

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in fusome

inferred from direct assay

(Lighthouse et al., 2008)

part_of plasma membrane proton-transporting V-type ATPase complex

inferred from mutant phenotype

(Allan et al., 2005)

Expression Deduced from Reporters

Reporter: P{PTT-GA}Vha16-1^CA06708

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

germarium

• fusome

(Lighthouse et al., 2008)

Reporter: PBac{544.SVS-1}Vha16-1^CPTI100041

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

Comment: adult brain cortex high expression in these neurons (too far from the fan-shaped body to be noduli)

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Vha16-1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Vha16-1

View exonic expression by tissue for Dmel\Vha16-1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44307/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262736

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004145

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262736

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3161

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 52 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Vha16-1

Transgenic constructs containing regulatory region of Vha16-1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

Df(2R)hfl-37

(Rendina et al., 2010, Rendina et al., 2010)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Vha16-1^CA06708

lethal, with Scer\GAL4^pnr-MD237

Vha16-1^GD17431

lethal | recessive

Vha16-1^EP2372

lethal - all die during embryonic stage

Vha16-1^P16

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vha16-1^GD17431

viable

viable, with Scer\GAL4^tin.CΔ4

Vha16-1^GD17431

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^pHCl-2.PR

Vha16-1^GD17431

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Vha16-1^EP2372

abnormal starvation stress response | adult stage

Vha16-1^m2

abnormal starvation stress response | adult stage | dominant

Vha16-1^EP2372

decreased cell death | oogenesis stage S13, with Scer\GAL4^GR1

Vha16-1^HMS02171

short lived

Vha16-1^m2

short lived | dominant

Vha16-1^EP2372

some die during embryonic stage

Vha16-1^P16

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vha16-1^GD17431

Phenotype manifest in

Allele

adult midgut

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Vha16-1^EP2372

dorsal appendage | oogenesis stage S14, with Scer\GAL4^GR1

Vha16-1^HMS02171

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Vha16-1^EY00180

embryonic/larval midgut | larval stage, with Scer\GAL4^lab.PH

Vha16-1^GD17431

macrochaeta

Malpighian tubule

Vha16-1^P16

nurse cell | oogenesis stage S13, with Scer\GAL4^GR1

Vha16-1^HMS02171

nurse cell | oogenesis stage S14 | increased number, with Scer\GAL4^GR1

Vha16-1^HMS02171

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\ATP6V0C

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Atp6v0c

14 of 14

Yes

Rnor\Atp5mc1

1 of 14

Rnor\Atp5mc2

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Atp6v0c

13 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\atp6v0c

3 of 13

Yes

Xtro\atp5mc3

1 of 13

Danio rerio (Zebrafish) (6)

Drer\atp6v0ca

14 of 14

Yes

Drer\atp6v0cb

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\vha-2

13 of 14

Yes

Cele\vha-3

13 of 14

Yes

Cele\vha-1

11 of 14

Yes

Cele\vha-4

2 of 14

Cele\Y82E9BR.3

1 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP000523

2 of 12

Yes

Agam\AgaP_AGAP009334

2 of 12

Yes

Agam\AgaP_AGAP002105

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\AT2G16510

12 of 13

Yes

Atha\AVA-P1

12 of 13

Yes

Atha\AVA-P2

12 of 13

Yes

Atha\AVA-P4

12 of 13

Yes

Atha\VHA-C3

12 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\VMA3

13 of 13

Yes

Scer\VMA11

7 of 13

Yes

Scer\VMA16

2 of 13

Scer\OLI1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\vma3

12 of 12

Yes

Spom\vma11

7 of 12

Yes

Spom\vma16

2 of 12

Escherichia coli (enterobacterium) (1)

Ecol\atpE

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Vha16-1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

13 of 13

11 of 13

11 of 13

10 of 13

4 of 13

4 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

epilepsy, early-onset, 3, with or without developmental delay

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ATP6V0C; ATPase H+ transporting V0 subunit c

model of early-onset epilepsy 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Vha16-1^GD4523

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Vha16-1^GD17431

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

ameliorates high grade glioma

modeled by Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Formica et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATP6V0C; ATPase H+ transporting V0 subunit c

14 of 14

108745

EPILEPSY, EARLY-ONSET, 3, WITH OR WITHOUT DEVELOPMENTAL DELAY; EPEO3

early-onset epilepsy 3

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Vha16-1 - pum

pull down, anti tag western blot

(Gerber et al., 2006)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, P23380 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262736

MIST (protein-protein) - An integrated Molecular Interaction Database

44307

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42B2-42B2

Sequence location

2R:6,625,242..6,633,090 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42B2-42B2

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

41-42

(Finbow, 1994.1.17)

42B1-42B2

(Dow, 1999)

42B1-42B2

(determined by in situ hybridisation)

(Finbow et al., 1994, Finbow et al., 1993)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

14814

y¹ w^67c23; P{EPgy2}EY00180

17244

w¹¹¹⁸; P{EP}Vha16-1^EP2372/CyO

65729

w¹¹¹⁸; PBac{IT.GAL4}Vha16-1^2177-G4

12594

w¹¹¹⁸; P{GT1}Vha16-1^BG01842a P{GT1}BG01842b

Kyoto

113272

w^*; P{GawB}Vha16-1^NP3427

103733

w^*; P{GawB}Vha16-1^NP0734 / CyO

112967

w^*; P{GawB}Vha16-1^NP2560 / CyO

115084

w¹¹¹⁸; PBac{544.SVS-1}Vha16-1^CPTI100041

115541

w¹¹¹⁸; PBac{681.P.FSVS-1}Vha16-1^CPTI002280

VDRC

v49291

P{GD17431}v49291

v104490

P{KK109332}VIE-260B

v49290

w¹¹¹⁸; P{GD17431}v49290

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (553)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH30178

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262736

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against Vha16 that is transfected into S2 treated with Listeria monocytogenes reveals Vha16 to be involved in Listeria monocytogenes entry and vacuolar escape.

(Cheng et al., 2005)

Vha16 has been cloned and sequenced.

(Finbow et al., 1994)

Vha16 plays a role in gap junctional communication in the follicles. Evidence suggests that the Vha16 gene product may double as a component of an ATP driven proton pump and a component of a passive, non-selective, channel.

(Finbow et al., 1993)

A Vha16 cDNA has been cloned and sequenced.

(Meagher et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: Vha16 CG3161

(Bayraktaroglu, 2000.4.13)

Source for merge of: Vha16 BcDNA:SD02875

(FlyBase, 1996-)

Source for merge of: Vha16-1 MRE15

(FlyBase Genome Annotators, 2011)

Additional comments

MRE15 is part of the 3' UTR of Vha16-1.

(FlyBase Genome Annotators, 2011)

MRE15 corresponds to a portion of the 3' UTR of Vha16-1.

(FlyBase Genome Annotators, 2011)

Source for merge of Vha16 BcDNA:SD02875 was a shared cDNA ( date:020730 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

ATP6V0C

(Adamson et al., 2011)

BcDNA:SD02875

CG3161

(Neuert et al., 2017, Butzlaff et al., 2015, Meadows, 2014.12.12, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Quinones-Coello, 2007, Saleh et al., 2006, Allan et al., 2005, Laviolette et al., 2005, Wang et al., 2004, Norga et al., 2003, Labourier et al., 2002, Fulton et al., 2001)

DucC

EP2372

MRE15

(FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)

V-ATPase

(Paraskevopoulos and McGuigan, 2021, Allan et al., 2002)

Vha1

(Laviolette et al., 2005)

Vha16

(Neuert et al., 2017, Butzlaff et al., 2015, Ashwal-Fluss et al., 2014, DeSalvo et al., 2014, Carreira et al., 2011, Reis et al., 2010, Lighthouse et al., 2008, Stofanko et al., 2008, Bai et al., 2007, Buszczak et al., 2007, Langille and Clark, 2007, Quinones-Coello, 2007, Saleh et al., 2006)

Vhac

v-ATPase

(Texada et al., 2020)

vha16

(Zoncu et al., 2011, Larsen et al., 2006, Cheng et al., 2005, Wang et al., 2004, Dow et al., 1997)

vha16-1

(Maruzs et al., 2023, Schotthöfer and Bohrmann, 2020, Lin et al., 2015, Wang et al., 2014, Chintapalli et al., 2013, Adamson et al., 2011, Allan et al., 2005, Stark et al., 2005, Dow, 1999)

Name Synonyms

Ductin

(Graham et al., 1997)

Ductin subunit C

Vacuolar H+ ATPase 16kD subunit

(Cammarato et al., 2011)

Vacuolar H+ ATPase 16kD subunit 1

Vacuolar H+ ATPase subunit 16-1

Vacuolar H+-ATPase c subunit

c subunit

(Dow et al., 1997)

ductin

(Bohrmann and Bonafede, 2000, Bohrmann and Braun, 1999, Bohrmann and Lammel, 1998, Guo et al., 1996)

ductin, vacuolar H(+)-ATPase subunit C proteolipid

(Finbow, 1994.1.17)

mRNA-like ncRNA in embryogenesis 15

Secondary FlyBase IDs

FBgn0004145
FBgn0011275
FBgn0015839
FBgn0033078
FBgn0044467
FBgn0046764
FBgn0086547

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 70 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44307

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P23380

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P23380

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P23380

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44307/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262736

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262736

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3161

MARRVEL_MODEL - MARRVEL (model organism gene)

44307

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262736

DroID - A comprehensive database of gene and protein interactions.

FBgn0262736

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Vha16-1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004145

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0262736

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3161

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0262736

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011025

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

44307

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (138)

The following external sites may use different assemblies or annotations than FlyBase.

-1.08

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