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FB2026_01
,
released March 12, 2026
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
HGScore = 62.35594
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Positive control.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
N-terminally truncated; does not contain binding sites for entire CCR4-NOT complex.
Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.
Not1 was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Not3 was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was live S2R+ cells; bait and prey produced from endogenous genes.