FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nxt1, sbr
FlyBase ID
FBig0000090671
Interaction Network
Interactions Browser links
Nxt1 network
sbr network
Reported Interactions
FBrf0200863-1.coIP.WB
Reference
Farny et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

p15

bait

V5 tag

sbr 

NXF1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Farny et al., 2008)

Prey expressed in transfected cells.

(Farny et al., 2008)
FBrf0218395-2899.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 
unspecified role
sbr 
unspecified role
sbr 
unspecified role
Nxt1 
unspecified role
Nxt1 
unspecified role
Nxt1 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 128.32624

FBrf0243126-15.coIP.WB
Reference
ElMaghraby et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

Nxt1

bait

GFP tag

sbr 

Nxf1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(ElMaghraby et al., 2019)
FBrf0243126-16.coIP.MS
Reference
ElMaghraby et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

Nxt1

bait

LAP tag or tdTomato-HA tag

sbr 

Nxf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(ElMaghraby et al., 2019)
FBrf0244040-12.XC
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

neutral component
sbr 

dNxf1

neutral component
sbr 
sbr 

dNxf1

neutral component
sbr 
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 domain
sufficient binding region
aa 359-612

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Zhao et al., 2019)
FBrf0244040-14.coIP.WB
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
Nxt1 

dNxt1

bait

GFP tag

sbr 

dNxf1

prey

Halo tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhao et al., 2019)
FBrf0244040-26.PD.MW
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

GST tag

sbr 
sbr 
Nxt1 

dNxt1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 domain
sufficient binding region
aa 359-544

coordinates relative to sbr-PA

sbr 
UBA domain
sufficient binding region
aa 601-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The sbr-Nxt1 interaction may not be direct as nxf2 was also added to the pull-down assay.

(Zhao et al., 2019)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhao et al., 2019)
FBrf0244040-28.CL.MS
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

neutral component
sbr 

dNxf1

neutral component
sbr 
sbr 

dNxf1

neutral component
sbr 
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Nxt1 

dNxt1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 and UBA domains
sufficient binding region
aa 359-672

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Zhao et al., 2019)
FBrf0244040-7.Y2H
Reference
Zhao et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sbr 

dNxf1

bait

fused to lexA DNA-binding domain

sbr 
Nxt1 

dNxt1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sbr 
NTF2 domain
sufficient binding region
aa 359-612

coordinates relative to sbr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. The presence of the sbr UBA domain decreased the sbr-Nxt1 interaction.

(Zhao et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhao et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)