Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000091008
Interaction Network
Interactions Browser links
osa network
brm network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0124978-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

Osa

bait
brm 

Brm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

osa coimmunoprecipitates brm and Snr1 (Brahma complex), but not Iswi or ash2.

osa exists in a subset of brm-containing complexes.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0124978-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

Brm

bait
osa 

Osa

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm coimmunoprecipitates osa and Snr1 (Brahma complex), but not Iswi.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0124978-5.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

Osa

bait
brm 

Brm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

FBrf0124978-8.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

Brm

bait
osa 

Osa

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

FBrf0127148-1.coIP.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
osa 

OSA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm, osa, Bap170, p400, mor, dalao, Bap60, Bap55, Snr1, Bap47 (actin) and Bap26 were identified as components of the Brahma complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before immunoprecipitation of brm.

FBrf0174961-1.coIP.MS
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
osa 

OSA

prey

21 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

FBrf0174961-13.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
osa 

OSA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0174961-18.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

OSA

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

osa can immunoprecipitate brm and mor, but not polybromo, suggesting that osa and polybromo exist in distinct Brahma complexes.

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0174961-21.CH.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

OSA

neutral component
osa 

OSA

neutral component
osa 

OSA

neutral component
brm 

BRM

neutral component
brm 

BRM

neutral component
brm 

BRM

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

osa is present with brm and mor in a distinct Brahma complex that lacks polybromo and Bap170.

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous gene.

Nuclear extract was subjected to several chromatographic steps to isolate brm-containing complexes.

FBrf0205136-11.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

BRM

bait
osa 

OSA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0205136-18.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

OSA

bait
brm 

BRM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0218395-4198.DPiM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 
unspecified role
brm 
unspecified role
brm 
unspecified role
osa 
unspecified role
osa 
unspecified role
osa 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 95.48328

FBrf0219981-17.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osa 

Osa

bait
brm 

Brm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)