Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Co-expression of hh or smoCT reduces interaction

The ci construct used contains Serine-to-Alanine mutations in three PKA phosphorylation sites to prevent proteolytic processing.

This interaction is inhibited by the co-expression of activated fu constructs.

Bait expressed in transiently transfected cells.

Prey expressed in transiently transfected cells.