FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ack, yki
FlyBase ID
FBig0000095699
Interaction Network
Interactions Browser links
Ack network
yki network
Reported Interactions
FBrf0218372-2.coIP.MS
Reference
Schoenherr et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ack 

Ack

bait

His tag, FLAG tag

yki 

Yorkie

prey

9 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cells.

(Schoenherr et al., 2012)

Prey endogenous to cell extract.

(Schoenherr et al., 2012)
FBrf0224345-178.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

Ack 

Ack

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0232944-4.PD.MS
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

yki 
Ack 

Ack

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Hu et al., 2016)
FBrf0232944-5.coIP.WB
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

Ack 

Ack

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hu et al., 2016)
FBrf0232944-8.coIP.WB
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ack 

Ack

bait

V5 tag

yki 

Yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)