FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cul3, rdx
FlyBase ID
FBig0000095837
Interaction Network
Interactions Browser links
Cul3 network
rdx network
Reported Interactions
FBrf0194555-5.coIP.WB
Reference
Zhang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
rdx 

HIB

bait

HA tag

rdx 
Cul3 

Cul3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rdx 
BTB domain
sufficient binding region
aa 180-374
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2006)
FBrf0194555-6.PD.A
Reference
Zhang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rdx 

HIB

bait

GST tag

rdx 
Cul3 

Cul3

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rdx 
BTB domain
sufficient binding region
aa 180-374
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Zhang et al., 2006)
FBrf0202727-3.Y2H
Reference
Arama et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3

bait

fused to GAL4 DNA-binding domain

rdx 

Spop

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Arama et al., 2007)

rdx interacts with somatic and testis-specific Cul-3 isoforms.

(Arama et al., 2007)

Bait was previously cloned EST (AT19493).

(Arama et al., 2007)

Prey derived from an adult Drosophila cDNA library.

(Arama et al., 2007)
FBrf0211875-5.coIP.WB
Reference
Kim et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rdx 

HIB/rdx

bait

His tag

Cul3 

Cullin3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Kim et al., 2010)
FBrf0221943-21.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
rdx 

Rdx

bait

FLAG tag

Cul3 

Cul3

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Zhang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)
FBrf0229291-1.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
rdx 

HIB

bait

Myc tag

Cul3 

Cul3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)
FBrf0229291-2.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
rdx 

HIB

bait
Cul3 

Cul3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Zhou et al., 2015)
FBrf0229291-5.coIP.WB
Reference
Zhou et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3

bait

HA tag

Cul3 
rdx 

HIB

prey

Myc tag

rdx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cul3 
BTB-binding domain
necessary binding region
aa 35-75

coordinates relative to Cul3-PA

rdx 
C-terminal region
sufficient binding region
aa 180-374

includes BTB domain, coordinates relative to rdx-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)