FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pzg, BEAF-32
FlyBase ID
FBig0000096061
Interaction Network
Interactions Browser links
pzg network
BEAF-32 network
Reported Interactions
FBrf0216935-4.coIP.WB
Reference
Gan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pzg 

Z4

bait
BEAF-32 

BEAF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell extract.

(Gan et al., 2011)

Estimate that ~50% of BEAF-32 protein coimmunoprecipitated with pzg protein.

(Gan et al., 2011)
FBrf0241977-1148.Y2H
Reference
Shokri et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pzg 

pzg

bait

fused to GAL4 DNA-binding domain

BEAF-32 

BEAF-32

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shokri et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Shokri et al., 2019)
FBrf0249398-2.coIP.WB
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait
pzg 

Z4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Melnikova et al., 2021)
FBrf0249398-3.Y2H
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BEAF-32 

BEAF-32

bait

fused to GAL4 DNA-binding domain

BEAF-32 
BEAF-32 
BEAF-32 
BEAF-32 
BEAF-32 
pzg 

Z4

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BEAF-32 
leucine zipper and BESS domains residues 200-283
mutation disrupting interaction
aa 200-283

coordinates relative to BEAF-32-PA

BEAF-32 
BESS domain residues 230-283
mutation decreasing interaction
aa 1-200

coordinates relative to BEAF-32-PA

BEAF-32 
BED and BESS domains residues 1-58 and 230-283
mutation disrupting interaction
aa 1-58 and 230-283

coordinates relative to BEAF-32-PA

BEAF-32 
BED and LZ domains residues 1-230
mutation disrupting interaction
aa 1-230

coordinates relative to BEAF-32-PA

BEAF-32 
BED domain residues 1-200
mutation decreasing interaction
aa 1-200

coordinates relative to BEAF-32-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2021)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2021)
FBrf0249398-5.Y2H
Reference
Melnikova et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pzg 

Z4

bait

fused to GAL4 DNA-binding domain

pzg 
pzg 
pzg 
pzg 
pzg 
BEAF-32 

BEAF-32

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pzg 
conservative domain residues 98-169
mutation disrupting interaction
aa 98-169

coordinates relative to pzg-PA

pzg 
zinc finger domains 3-7 residues 293-997
mutation disrupting interaction
aa 293-997

coordinates relative to pzg-PA

pzg 
conservative and zinc finger domains 1-3; residues 1-325
sufficient binding region
aa 1-325

coordinates relative to pzg-PA

pzg 
zinc finger domains 1-2 residues 238-293
mutation disrupting interaction
aa 238-293

coordinates relative to pzg-PA

pzg 
zinc finger domain 3 residues 293-325
mutation decreasing interaction
aa 293-325

coordinates relative to pzg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Melnikova et al., 2021)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)