FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ci, nej
FlyBase ID
FBig0000097105
Interaction Network
Interactions Browser links
ci network
nej network
Reported Interactions
FBrf0093058-1.LPD
Reference
Akimaru et al., 1997
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 12embryonic stage 13
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

GST tag

nej 
ci 

Ci

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
region
sufficient binding region
aa 781-1159
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system (prey from embryo cDNA expression library).

(Akimaru et al., 1997)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Akimaru et al., 1997)
FBrf0093058-2.Y2H
Reference
Akimaru et al., 1997
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 11blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

fused to LexA DNA-binding domain

nej 
ci 

Ci

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
region
sufficient binding region
aa 825-1043
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Akimaru et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Akimaru et al., 1997)
FBrf0093058-3.PD.MW
Reference
Akimaru et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

GST tag

ci 

Ci

prey

[35]S label

ci 
ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ci 
Ci activation domain
sufficient binding region
aa 1020-1160
ci 
Ci activation domain
necessary binding region
aa 1020-1160
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Akimaru et al., 1997)
FBrf0125204-1.Y2H
Reference
Chen et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

fused to LexA DNA-binding domain

nej 
ci 

CI

prey

fused to GAL4 activation domain

ci 
ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
CI interaction domain
sufficient binding region
aa 835-1043

coordinates relative to nej-PA

ci 
C terminal region
sufficient binding region
aa 918-1377

coordinates relative to ci-PA

ci 
CBP binding domain
necessary binding region
aa 1020-1160

coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2000)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Chen et al., 2000)
FBrf0125204-2.Y2H
Reference
Chen et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

fused to LexA DNA-binding domain

nej 
ci 

CI

prey

fused to GAL4 activation domain

ci 
ci 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
CI interaction domain
sufficient binding region
aa 835-1043

coordinates relative to nej-PA

ci 
CBP binding domain
sufficient binding region
aa 984-1377

coordinates relative to ci-PA

ci 
CBP binding domain
mutation decreasing interaction
aa 1040aa 1054aa 1082aa 1087aa 1088aa 1089aa 1145aa 1010,1123aa 1047,1151aa 1077,1151aa 1083,1089,1146aa 1087,1096,1098

I1040T, coordinates relative to ci-PA

F1054L, coordinates relative to ci-PA

L1082H, coordinates relative to ci-PA

E1087G, coordinates relative to ci-PA

M1088T, coordinates relative to ci-PA

L1089P, coordinates relative to ci-PA

D1145G, coordinates relative to ci-PA

P1010A, H1123R, coordinates relative to ci-PA

D1047N, K1151R, coordinates relative to ci-PA

D1077G, K1151R, coordinates relative to ci-PA

I1083T, L1089P, I1146M, coordinates relative to ci-PA

E1087G, K1096R, D1098G, coordinates relative to ci-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2000)

Split two-hybrid system: yeast LexA-BD/GAL4-AD, "The split-hybrid system is a two-component reporter system that converts the disruption of a protein-protein interaction into a positive selection."

(Chen et al., 2000)
FBrf0232221-4.coIP.WB
Reference
Han et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ci 

ci

bait

HA tag

nej 

nej

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2015)

Coexpression of Su(fu) inhibits the ci-nej interaction. This inhibition is dependent on the C-terminus of ci.

(Han et al., 2015)
FBrf0245753-8.coIP.WB
Reference
Han et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
ci 

Ci

bait

Myc tag

nej 

dCBP

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ci-nej interaction assessed with a form of ci with PKA site mutations S838A, S856A, S892A. Coexpression of Su(fu) inhibits the ci-nej interaction.

(Han et al., 2019)

Interaction in vitro; bait derived from extract of transfected S2 cells; prey produced as a recombinant fusion protein in a baculovirus and Sf9 cell system.

(Han et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)