FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, Tgi
FlyBase ID
FBig0000097501
Interaction Network
Interactions Browser links
yki network
Tgi network
Reported Interactions
FBrf0221730-3.coIP.WB
Reference
Koontz et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Tgi 

Tgi

bait

Myc tag

Tgi 
yki 

Yki

prey

HA tag

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tgi 
PPxY motif 1
mutation disrupting interaction
aa 244

Y244A in PPPY motif

yki 
region
mutation disrupting interaction
aa 65

P65L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Koontz et al., 2013)

Of three PPxY motifs in CG10741 (Tgi), only the first (PPPY) is critical for its interaction with yki.

(Koontz et al., 2013)

yki inhibits the interaction of CG10741 (Tgi) with sd.

(Koontz et al., 2013)
FBrf0224345-182.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

Tgi 

CG10741

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0224710-10.Y2H
Reference
Guo et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

fused to GAL4 DNA-binding domain

Tgi 

SdBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Guo et al., 2013)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Guo et al., 2013)
FBrf0224710-11.PD.WB
Reference
Guo et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

Tgi 

SdBP

prey

FLAG tag

Tgi 
Tgi 
Tgi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tgi 
PPXY motif 1
mutation decreasing interaction
aa 88-91

coordinates relative to the 382 residue Tgi-PB isoform

Tgi 
PPXY motif 2
mutation decreasing interaction
aa 273-276

coordinates relative to the 382 residue Tgi-PB isoform

Tgi 
PPXY motif 3
mutation decreasing interaction
aa 283-286

coordinates relative to the 382 residue Tgi-PB isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell lysate.

(Guo et al., 2013)

Mutation of all 3 PPXY motifs in Tgi disrupts binding to yki completely.

(Guo et al., 2013)
FBrf0224710-12.PD.WB
Reference
Guo et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Tgi 

SdBP

bait

GST tag

yki 

Yki

prey

Myc tag

yki 
yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domain 1
mutation decreasing interaction
yki 
WW domain 2
mutation decreasing interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell lysate.

(Guo et al., 2013)

Mutation of both WW domains in yki disrupts binding to Tgi completely.

(Guo et al., 2013)

Mutation of the Tgi TDU domains does not affect the interaction with yki.

(Guo et al., 2013)
FBrf0224710-6.coIP.WB
Reference
Guo et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

Tgi 

SdBP

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sequential coimmunoprecipitation, first of FLAG-Tgi, then of Myc-yki, in the presence of HA-sd, suggests that sd, yki and Tgi form a ternary complex.

(Guo et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guo et al., 2013)
FBrf0224710-7.coIP.WB
Reference
Guo et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Tgi 

SdBP

bait

FLAG tag

Tgi 
Tgi 
Tgi 
yki 

Yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tgi 
PPXY motif 1
mutation decreasing interaction
aa 88-91

coordinates relative to the 382 residue Tgi-PB isoform

Tgi 
PPXY motif 2
mutation decreasing interaction
aa 273-276

coordinates relative to the 382 residue Tgi-PB isoform

Tgi 
PPXY motif 3
mutation decreasing interaction
aa 283-286

coordinates relative to the 382 residue Tgi-PB isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guo et al., 2013)

Mutation of all 3 PPXY motifs in Tgi disrupts binding to yki completely.

(Guo et al., 2013)

Mutation of the Tgi TDU domains does not affect the interaction with yki.

(Guo et al., 2013)

Sequential coimmunoprecipitation, first of FLAG-Tgi, then of Myc-yki, in the presence of HA-sd, suggests that sd, yki and Tgi form a ternary complex.

(Guo et al., 2013)
FBrf0238965-1.CH.MW
Reference
Nyarko, 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

neutral component
yki 

Yki

neutral component
yki 

Yki

neutral component
Tgi 

Tgi

neutral component
Tgi 

Tgi

neutral component
Tgi 

Tgi

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Nyarko, 2018)
FBrf0238965-2.ITC
Reference
Nyarko, 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tgi 

Tgi

bait

His tag

Tgi 
Tgi 
yki 

Yki

prey

His tag

yki 
yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW1 domain
sufficient binding region
aa 232-306
yki 
WW2 domain
sufficient binding region
aa 303-352
Tgi 
P1 domain
sufficient binding region
QRASPPPPYREPLP
Tgi 
P3 domain
sufficient binding region
TPPPPPPAYGIAGT
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system or prey peptides produced commercially.

(Nyarko, 2018)

Kd = 6.5uM

(Nyarko, 2018)
FBrf0238965-3.NMR
Reference
Nyarko, 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

neutral component
yki 

Yki

neutral component
yki 
yki 

Yki

neutral component
yki 
Tgi 

Tgi

neutral component

[15]N label

Tgi 
Tgi 
Tgi 

Tgi

neutral component

[15]N label

Tgi 

Tgi

neutral component

[15]N label

Tgi 
Tgi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
tandem WW domains
sufficient binding region
aa 232-352
Tgi 
P1 domain
sufficient binding region
aa 1-97
Tgi 
P2 and P3 domain
sufficient binding region
aa 258-382
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Nyarko, 2018)
FBrf0245637-1.PD.MS
Reference
Vissers et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tgi 

Tgi

bait

SBP tag

yki 

Yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vissers et al., 2020)
FBrf0247927-14.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

Tgi 

Tgi

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)

The Yki2/yki-PF isoform including exon 3 interacted more strongly with Tgi than an isoform lacking exon 3.

(Srivastava et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)