FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc42, mbt
FlyBase ID
FBig0000097899
Interaction Network
Interactions Browser links
Cdc42 network
mbt network
Reported Interactions
FBrf0155712-1.coIP.WB
Reference
Schneeberger and Raabe, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbt 

Mbt

bait

HA tag

mbt 
Cdc42 

Cdc42

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbt 
PBD domain
mutation disrupting interaction
aa 19-21

H19L, H21L double mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schneeberger and Raabe, 2003)

mbt binds Cdc42, but not Rac1 or Rho1.

(Schneeberger and Raabe, 2003)
FBrf0155712-2a.PD.WB
Reference
Schneeberger and Raabe, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

mbt 

Mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mbt interacts with the activated, GTP-loaded form of Cdc42, but not GDP-loaded Cdc42.

(Schneeberger and Raabe, 2003)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from HEK293 cell extract.

(Schneeberger and Raabe, 2003)
FBrf0221273-1.coIP.WB
Reference
Melzer et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbt 

Mbt

bait

Myc tag

Cdc42 

Cdc42

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mbt interacts with Cdc42 but not Rac1, Rac2, Mtl, Rho1 or RhoL in this assay.

(Melzer et al., 2013)

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Melzer et al., 2013)
FBrf0260768-6.PD.WB
Reference
Rötte et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

mbt 

MBT

prey

GFP tag

mbt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbt 
CRIB binding domain
sufficient binding region
aa 11-49

coordinates relative to mbt-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct.

(Rötte et al., 2024)

Cdc42 interacts equally well with the CRIB binding domain of mbt when preloaded with GDP or GTPγS.

(Rötte et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)