Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutations of both Tondu domains in CG10741 (Tgi) disrupts the interaction with sd completely.

yki inhibits the interaction of CG10741 (Tgi) with sd.

Interaction in vitro; bait produced from transfected construct in S2R+ cell line; prey produced as a recombinant fusion protein in bacterial system.

yki inhibits the interaction of CG10741 (Tgi) with sd in vitro.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Mutation of both TDU domains in Tgi disrupts binding to sd completely.

Mutation of the Tgi PPXY motifs does not affect the interaction with sd.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Mutation of both TDU domains in Tgi disrupts binding to sd completely.

The Tgi-sd interaction is stimulated by the presence of yki. However, if the Tgi PPXY motifs are disabled, then yki inhibits the Tgi-sd interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutation of both TDU domains in Tgi disrupts binding to sd completely.

Mutation of the Tgi PPXY motifs does not affect the interaction with sd.

Sequential coimmunoprecipitation, first of FLAG-Tgi, then of Myc-yki, in the presence of HA-sd, suggests that sd, yki and Tgi form a ternary complex.

Increased expression of ci has no effect on binding between hpo and sav.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Increased expression of yki results in a decrease in binding between sd and Tgi in a dose-dependent manner.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Coexpression of the Yki2/yki-PF isoform including exon 3 enhanced the sd-Tgi interaction more strongly than an isoform lacking exon 3. This enhancement is dependent on the two WW domains.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

570 +/- 50; 3.0 +/- 0.4 nM

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.