Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Both 14-3-3ε and 14-3-3ζ are coexpressed with yki. Co-expression of wts, hpo and sav which increase yki phosphorylation increases the yki-14-3-3 interactions.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The yki-14-3-3ε interaction is strengthened by cotransfection of hpo, wts and mats.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

This system was used in an RNAi screen to identify genes that perturb the yki-14-3-3ε interaction.

TEV complementation assay: interaction of bait and prey reconstitutes a functional TEV protease, which in turn cleaves a chimeric fusion of GAL4 and estrogen receptor (ERT2) ligand binding domain. In the absense of TEV activity, the ERT2 ligand binding domain restricts the chimeric protein to the cytosol. Upon cleavage, the free GAL4-VP16 portion of the chimera can activate a UAS-reporter.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

HA tagged versions of both 14-3-3ε and 14-3-3ζ were coexpressed with Myc tagged yki for immunoprecipitation assays. Curated as two interactions. Interaction increased upon coexpression of CkIIα and CkIIβ or hpo and wts.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

HA tagged versions of both 14-3-3ε and 14-3-3ζ were coexpressed with Myc tagged yki for immunoprecipitation assays. Curated as two interactions. Interaction increased upon coexpression of CkIIα and CkIIβ or hpo and wts.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.

No difference in yki-14-3-3ε interaction with different yki isoforms including or excluding exon 3. The specific 14-3-3 gene used was not specified so interactions were captured for both 14-3-3ε and 14-3-3ζ.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.