Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult fly extract.
Positive control.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of SF4 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
Y572G
L578G, Y580G double mutation
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 cell system; prey produced and labeled by in vitro translation.
Source was cell extract of Sg4 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; proteins produced as a recombinant fusion proteins in a baculovirus and insect cell system.
E886R, F893R, H897R, F945E, A965E, F979E sextuple mutation
Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
E886R, F893R, H897R, F945E, A965E, F979E sextuple mutation
Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.
Positive control.
Source was cell extract of S2-DRSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.
K532R,K539R,K541R,K545R,K694R,K706R,K715R,K773R,K776R
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was embryos of engineered fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.
Source was ovaries of engineered fly line; bait produced from tagged endogenous gene; prey produced from endogenous gene.
