Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from 293T cell extract.
Two-hybrid system: yeast DNA BD/Gal4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system; enzyme target produced as a recombinant fusion protein in bacterial system.
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
Positive control.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
