FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, ex
FlyBase ID
FBig0000098828
Interaction Network
Interactions Browser links
yki network
ex network
Reported Interactions
FBrf0207465-1.coIP.MS
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

yki 

Yki

prey

14 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Badouel et al., 2009)
FBrf0207465-2.coIP.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
yki 

Yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C-terminal region
sufficient binding region
aa 748-1427

"CT" construct, coordinates relative to ex-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Badouel et al., 2009)
FBrf0207465-3.coIP.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait
ex 

Ex

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Badouel et al., 2009)
FBrf0207465-4.PD.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

yki 
yki 
yki 
ex 

Ex

prey

His tag

ex 
ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C-terminal region
sufficient binding region
aa 748-1427

"CT" construct, coordinates relative to ex-PA

ex 
PPxY motifs 1-3
mutation disrupting interaction
aa 789-1206

Y789A, Y847A, and Y1206A, "3Y" construct, coordinates relative to ex-PA

yki 
C-terminal WW domains
sufficient binding region

"C" construct

yki 
WW domain 1 residues W292 and P295
mutation decreasing interaction
aa 292-295

W292A and P295A, "ΔWW1" construct, coordinates relative to yki-PH

yki 
WW domain 2 residues W361 and P364
mutation decreasing interaction
aa 361-364

W361A and P364A, "ΔWW2" construct, coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Badouel et al., 2009)

Mutation of both yki WW domains completely disrupts ex binding. yki phosphorylation at Ser168 is dispensable for ex binding.

(Badouel et al., 2009)
FBrf0207465-5.coIP.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait
ex 

Ex

prey

FLAG tag

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
PPxY motifs 1-3
mutation disrupting interaction
aa 789-1206

Y789A, Y847A, and Y1206A, "3Y" construct, coordinates relative to ex-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Badouel et al., 2009)
FBrf0207465-6.coIP.WB
Reference
Badouel et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

ex 

Ex

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Badouel et al., 2009)
FBrf0215087-1.coIP.WB
Reference
Oh et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

V5 tag

yki 
ex 

Ex

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
mutation disrupting interaction
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh et al., 2009)
FBrf0224345-193.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

ex 

ex

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0232944-9.coIP.WB
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

ex 

Ex

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The yki-ex interaction is reduced upon coexpression of active Ack (but not a kinase dead form).

(Hu et al., 2016)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu et al., 2016)
FBrf0247927-11.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

ex 

Ex

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Yki2/yki-PF isoform including exon 3 interacted more strongly with hpo than an isoform lacking exon 3.

(Srivastava et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)
FBrf0253974-5.coIP.WB
Reference
Wang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ex 

ex

bait

FLAG tag

ex 
yki 

yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
PPPY motifs
necessary binding region
unspecified

Y amino acids in the 3 PPPY yki binding motifs were mutated to A; coordinates relative to ex-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2022)
FBrf0253974-6.coIP.WB
Reference
Wang et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Myc tag

ex 

ex

prey

FLAG tag

ex 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
PPPY motifs
necessary binding region
unspecified

Y amino acids in the 3 PPPY yki binding motifs were mutated to A; coordinates relative to ex-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)