FB2021_02, released April 13, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
chico, 14-3-3ε
FlyBase ID
FBig0000098937
Interaction Network
Interactions Browser links
chico network
14-3-3ε network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204162-1.coIP.MS
Reference
Pflieger et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
chico 

Chico

bait

HA tag

14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The enrichment of candidate chico-interacting proteins in control versus experimental samples was corrected for relative protein abundance in the lysates. Only three chico-interacting candidates were considered to be enriched above the threshold expected for abundant contaminant proteins: 14-3-3ε, 14-3-3ζ and InR.

(Pflieger et al., 2008)

The chico-14-3-3ε interaction is increased upon insulin stimulation.

(Pflieger et al., 2008)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Pflieger et al., 2008)
FBrf0216604-5.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
chico 

chico

bait

GFP tag

14-3-3ε 

14-3-3epsilon

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0230883-11.coIP.MS
Reference
Breitkopf et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
chico 

chico

bait

TAP tag

14-3-3ε 

14-3-3 protein epsilon

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding of 14-3-3ε to chico doubles upon insulin stimulation of cells.

(Breitkopf et al., 2016)
FBrf0233454-130.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
chico 

chico

bait

TAP tag

14-3-3ε 

14-3-3epsilon

prey

SAINT score = 0.9863

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in two of three conditions tested: uninduced cells and 10 minutes after insulin stimulation.

(Vinayagam et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)