Fmr1, Tdrd3 Physical Interaction report

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Tdrd3

dTDRD3

bait

FLAG tag

Fmr1

dFMR1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xu et al., 2013)

FBrf0223174-6.coIP.WB

Reference

Xu et al., 2013

Description

physical association

Assay

anti tag western blot

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Fmr1

dFMR1

bait

Tdrd3

dTDRD3

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Xu et al., 2013)

FBrf0240783-14.coIP.WB

Reference

Lee et al., 2018

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Identification by mass spectrometry

Participants

Corresponds to

Reported as

Role

Note

Fmr1

FMRP

bait

Tdrd3

TDRD3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lee et al., 2018)

FBrf0240783-18.coIP.MS

Reference

Lee et al., 2018

Description

physical association

Assay

coimmunoprecipitation

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Tdrd3

TDRD3

bait

Fmr1

FMRP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene or transfected construct; prey produced from endogenous gene.

(Lee et al., 2018)

FBrf0240783-2.coIP.WB

Reference

Lee et al., 2018

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Tdrd3

TDRD3

bait

Tdrd3

Fmr1

FMRP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Tdrd3

interaction domain

sufficient binding region

aa 501-836aa 1-720

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lee et al., 2018)

FBrf0240783-25.coIP.MS

Reference

Lee et al., 2018

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

Cell line used

cosedimentation through density gradient

Participants

Corresponds to

Reported as

Role

Note

Fmr1

FMRP

bait

Tdrd3

TDRD3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lee et al., 2018)

FBrf0259913-1.CS.WB

Reference

Ahmad et al., 2016

Description

physical association

Assay