FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, 14-3-3ζ
FlyBase ID
FBig0000100530
Interaction Network
Interactions Browser links
yki network
14-3-3ζ network
Reported Interactions
FBrf0202397-1.coIP.WB
Reference
Dong et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

HA tag

yki 
14-3-3ζ 

14-3-3ζ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
14-3-3 binding motif
mutation disrupting interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dong et al., 2007)
FBrf0204358-2.coIP.WB
Reference
Oh and Irvine, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

V5 tag

yki 

Yki

prey

FLAG tag

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
interaction domain
mutation disrupting interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh and Irvine, 2008)

Both 14-3-3ε and 14-3-3ζ are coexpressed with yki. Co-expression of wts, hpo and sav which increase yki phosphorylation increases the yki-14-3-3 interactions.

(Oh and Irvine, 2008)
FBrf0209792-2.coIP.WB
Reference
Ren et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

yki 
14-3-3ζ 

14-3-3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
14-3-3 predicted binding site residue S168
mutation disrupting interaction
aa 168

S168A, coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ren et al., 2010)

The yki-14-3-3ζ interaction is strengthened by cotransfection of hpo, wts and mats.

(Ren et al., 2010)
FBrf0224345-177.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

14-3-3ζ 

14-3-3zeta

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0226836-2.coIP.WB
Reference
Hu et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3ζ

bait

HA tag

yki 

yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu et al., 2014)

HA tagged versions of both 14-3-3ε and 14-3-3ζ were coexpressed with Myc tagged yki for immunoprecipitation assays. Curated as two interactions. Interaction increased upon coexpression of CkIIα and CkIIβ or hpo and wts.

(Hu et al., 2014)
FBrf0226836-4.coIP.WB
Reference
Hu et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Myc tag

14-3-3ζ 

14-3-3ζ

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu et al., 2014)

HA tagged versions of both 14-3-3ε and 14-3-3ζ were coexpressed with Myc tagged yki for immunoprecipitation assays. Curated as two interactions. Interaction increased upon coexpression of CkIIα and CkIIβ or hpo and wts.

(Hu et al., 2014)
FBrf0233262-68.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

yki 
14-3-3ζ 

14-3-3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
C-terminal region
sufficient binding region
aa 56-418

coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2016)

Positive control.

(Wang et al., 2016)
FBrf0233262-9.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
14-3-3ζ 

14-3-3

bait

HA tag

yki 

Yki

prey

Myc tag

yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
C-terminal region
sufficient binding region
aa 56-418

coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2016)

Positive control.

(Wang et al., 2016)
FBrf0247927-7.coIP.WB
Reference
Srivastava et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Firefly Luciferase tag

14-3-3ζ 

14.3.3

prey

Renilla Luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

No difference in yki-14-3-3ζ interaction with different yki isoforms including or excluding exon 3. The specific 14-3-3 gene used was not specified so interactions were captured for both 14-3-3ε and 14-3-3ζ.

(Srivastava et al., 2021)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Srivastava et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)