Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Positive control.
Interaction in vitro; Diap1 produced as a recombinant fusion proteins in bacterial system; hid peptide chemically synthesized.
Kd = 0.76uM
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.
Interaction in vitro; bait peptide commercially synthesized; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait peptide commercially synthesized; prey derived from S2 cell extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was S2R+ cell line; bait produced from tagged transfected construct; prey produced from transfected construct.
Prey identification based on comigration of the far western band with band detected by anti-W western blot.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from mutant or wild-type larval imaginal disc and brain extract.
