FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
gw, Patr-1
FlyBase ID
FBig0000101230
Interaction Network
Interactions Browser links
gw network
Patr-1 network
Reported Interactions
FBrf0212181-1.coIP.WB
Reference
Jäger and Dorner, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gw 

gw

bait

HA tag

gw 
Patr-1 

Patr-1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
C-terminal domain
sufficient binding region
aa 1095-1384
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jäger and Dorner, 2010)
FBrf0222534-1.coIP.WB
Reference
Barišić-Jäger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Patr-1 

Hpat

bait
gw 

GW182

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)
FBrf0222534-3.coIP.WB
Reference
Barišić-Jäger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait
Patr-1 

Hpat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)
FBrf0222534-7.coIP.WB
Reference
Barišić-Jäger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

TAP tag

Patr-1 

Hpat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)

Sequential affinity purification suggests that gw, AGO1 and Patr-1 exist in complex.

(Barišić-Jäger et al., 2013)

The interaction of gw with Patr-1 is resistant to RNaseA treatment.

(Barišić-Jäger et al., 2013)

Purified AGO1 complexes were subjected to further purification of tagged gw protein.

(Barišić-Jäger et al., 2013)
FBrf0222534-9.coIP.WB
Reference
Barišić-Jäger et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

HA tag

Patr-1 

Hpat

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Barišić-Jäger et al., 2013)

The interaction of Patr-1 with gw is not affected by knockdown of Ge-1 or pcm.

(Barišić-Jäger et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)