Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Pak binds to Rac1 and Cdc42, but not to Rho, Ras or Rab proteins tested.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected COS-7 cell extract.

Pak interacts specifically with GTP-bound Rac1, but not the GDP-bound form.

Pak interacts with Rac1 and Rac2, but not mtl.

GST-tagged Rac was preloaded with GDP or non-hydrolysable GTPγS.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Pak interacts with Rac1, but not mtl.

Two-hybrid system: yeast LexA-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Binding of PlexB and Pak to active Rac1 are mutually exclusive.

Two-hybrid system: yeast LexA-BD/B42-AD

Pak interacts with Rac1 and Rac2 but not Mtl in this assay.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Cells were treated with the robo ligand sli for 10 minutes before the pull down assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

The Pak-Rac1 interaction is greatly increased when cells express a functional robo receptor and are treated with the robo ligand sli.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293 cell extract.

Vav acts as an activating GEF for Rac1.

Pak pulls down activated Rac1 (Rac1-GTP);transfected wildtype trc suppresses activated Rac1 pull down.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected constructs expressing wt trc or a dominant-negative Trc mutant (K112A).