FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nerfin-1, sd
FlyBase ID
FBig0000101587
Interaction Network
Interactions Browser links
nerfin-1 network
sd network
Reported Interactions
FBrf0227292-433.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
sd 

FBgn0003345

unspecified role
sd 

FBgn0003345

unspecified role
sd 

FBgn0003345

unspecified role
nerfin-1 

FBgn0028999

unspecified role
nerfin-1 

FBgn0028999

unspecified role
nerfin-1 

FBgn0028999

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 35.400413

(Rhee et al., 2014)

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)
FBrf0240545-1.PD.WB
Reference
Vissers et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

SBP tag

nerfin-1 

Nerfin-1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Vissers et al., 2018)
FBrf0240545-2.coIP.WB
Reference
Vissers et al., 2018
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

GFP tag

nerfin-1 

Nerfin-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval braind of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vissers et al., 2018)
FBrf0241879-1.coIP.WB
Reference
Guo et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
nerfin-1 

Nerfin-1

bait

FLAG tag

nerfin-1 
nerfin-1 
sd 

Sd

prey

Myc tag

sd 
sd 
sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
N-terminal domain
necessary binding region
unspecified
sd 
TEA domain residue 108
mutation disrupting interaction
aa 108

Y108A

sd 
TEA domain residue 130
mutation disrupting interaction
aa 130

L130G

nerfin-1 
C-terminal domain
necessary binding region
unspecified
nerfin-1 
ZF1 domain
mutation disrupting interaction
unspecified

two Cys residues mutated to Ala in ZF1.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guo et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)